霉酚酸对大鼠肺微血管内皮细胞增殖及分泌内皮素-1白细胞介素-6的影响  

Effects of mycophenolic acid on the proliferation of rat pulmonary microvascular endothelial cells and the secretion of endothelin-1 and interleukin-6

作　　者：李海云[1] 郑毅[1] 

机构地区：[1]首都医科大学附属北京朝阳医院风湿免疫科,100020

出　　处：《中华风湿病学杂志》2014年第2期114-117,I0002,共5页Chinese Journal of Rheumatology

基　　金：国家自然科学基金（30872329）

摘　　要：【摘要】目的探讨霉酚酸对大鼠肺微血管内皮细胞（PMVECs）增殖及其分泌内皮素-1、IL-6的影响。方法大鼠PMVECs培养采用组织贴块法。细胞学实验分5组：对照组、低浓度组、中浓度组、高浓度组和极高浓度组，霉酚酸的终浓度分别为：0，0．1，1，10，100μmol／L。检测大鼠PMVECs增殖采用CCK．8（cellcountingkit．8）的方法。检测细胞上清液内皮素-1、IL-6的浓度采用放射免疫法，多组间比较采用单因素方差分析。结果培养的大鼠PMVECs呈梭形或多角形生长，第Ⅷ因子免疫组织化学染色阳性。各组在450nm的吸光度（A）值：加霉酚酸24h后，从对照组到极高浓度组分别为1．15±0．20，1．20±0．13，0．88±0．20，0．62±0．05和0．56±0．10，除对照组和低浓度组（P=0．388）、高浓度组与极高浓度组（P=0．292）间差异无统计学意义外，其余各组间差异均有统计学意义（P均〈0．01）；加霉酚酸48h后，从对照组到极高浓度组分别为2．61±O．21，2．52±0．23，1．99±0．17，0．84±0．16和0．65±0．12，除对照组和低浓度组间（P=-0．094）差异无统计学意义外，其余各组间差异均有统计学意义（P〈0．01）；加霉酚酸72h后，从对照组到极高浓度组分别为2．62±0．24，2．62±0．19，2．37±0．38，0．79±0．17和0．61±0．17，除对照组与低浓度组间（P=-0．931）差异无统计学意义外，其余各组间差异均有统计学意义（P均〈0.05）。加入霉酚酸48h后，5组细胞上清液内皮素-1的浓度，除对照组和低浓度组（降0．156）、高浓度组与极高浓度组间（P=O．262）差异无统计学意义外，其余各组间差异均有统计学意义（P均〈0．01）。加霉酚酸48h后，5组细胞上清液中IL-6的浓度差异均无统计学意义（P〉0．05）。结论霉酚酸能够抑制胎牛血清诱导的大鼠PMVECs的增殖及其分泌的内皮素-1，且呈浓度依赖性�Objective To investigate the effect of mycophenolic acid （MPA） on the proliferation of rat pulmonary microvascular endothelial cells （PMVECs） and the effects of MPA on the secretion of endothelin-1 （ET-1） and interleukin-6 （IL-6）. Methods PMVECs were cultured by tissue-sticking method. Cells were divided into 5 groups, named as the control group, low-dose MPA group, moderate-dose MPA group, high- dose MPA group and super high-dose MPA group, according to the final concentration of MPA （0, 0.1, 1, l0 and 100 Ixmol/L, respectively）. The proliferation of rat PMVECs was detected by cell counting kit-8 （CCK-8）. The ET-1 and IL-6 secretion were assessed by radio-immunoassay. One-way analysis of variance was used to detect the differences between the group means. Results The cultured PMVECs of rats in vitro showed fusiform or polygonal in shape, and the expressed factor V~ associated antigen stain was positive. At 24 h, the A values of the 5 groups （from control group to super high-dose MPA group） were 1.15±0.20, 1.20±0.13, 0.88±0.20, 0.62±0.05 and 0.56±0.10, respectively, there was no significant difference in the A values on 450 nm between the control group and the low-dose MPA group （P=0.388）, as well as the high-dose MPA group and super high-dose MPA group （P=0.292）, but there were significant differences between any other two groups （P〈0.01）. At 48 h, the A values of the 5 groups （from control group to super high-dose MPA group） were 2.61±0.21, 2.52±0.23, 1.99±0.17, 0.84±0.16 and 0.65±0.12, respectively, there was no significant difference in the A values on 450 nm between the control group and low-dose MPA group （P=0.094）, but there were significant differences in the A values between any other two groups （P〈0.01）. At 72 h, the A values of the 5 groups （from control group to super high-dose MPA group） were 2.62±0.24, 2.62±0.19, 2.37± 0.38, 0.79±0.17 and 0.61±0.17, respectively, there was no significant difference in the A values on 450 nm b

关 键 词：霉酚酸 高血压 肺性 大鼠 白细胞介素-6 

分 类 号：R544.1[医药卫生—心血管疾病]