川南地区汉族人群D1S80基因座多态性分析  

Polymorphism analysis of D1S80 locus in Chinese Han population in Southern Sichuan

作　　者：张天丹[1] 杨曼曼[1] 杨璐全[1] 邹永林[2] 傅俊江[1,3] 

机构地区：[1]泸州医学院医学基础研究中心表观遗传与肿瘤重点实验室,四川泸州646000 [2]重庆市公安局刑警总队技术处,重庆400021 [3]湖南省天衡司法鉴定所,湖南长沙410008

出　　处：《中国医药科学》2014年第1期9-11,共3页China Medicine And Pharmacy

基　　金：国家自然科学基金(81172049;30371493);四川省高校创新团队基金(13TD0032)部分资助项目

摘　　要：目的了解川南地区汉族人群DIS80位点群体遗传多态性。方法采用PCR结合聚丙烯酰胺PAGE电泳及高灵敏度银染技术，即Amp—FLP方法对川南地区120名汉族无血缘关系个体D1S80位点进行多态性分析。结果在所调查的120名无关个体样本中，观察到D1S80基因座有18个等位基因，基因频率分布在0．004166667～0．1958333333之间，杂合度为0．7869，个体识别力为0．87，非父排除率为0．8152，其基因型频率分布符合Hardy—Weinberg平衡法则。结论D1S80基因座的PCR分型具有较好的准确性和灵敏度，为法医学个体识别和亲权鉴定、遗传病基因链锁分析等的研究及应用提供了川南地区有用的遗传信息。Objective To study the population genetic polymorphism of D1S80 locus in Han population in Southern Sichuan. Methods PCR amplification, electrophoresis of PAGE and high sensitive silver staining method, namely Amp-FLP, were performed for D1SSO loci by polymorphic analysis in 120 unrelated Han individuals from Southern Sichuan. Results From survey of 120 unrelated individuals for D1S80 loci, 18 alleles are observed. Gene frequency is located between 0.004166667 and 0.1958333333,heterozygosity is 0.7869, discrimination power is 0.87,exclusion power is 0.8152,which is in accordance with Hardy-Weinberg balance rule for the genotype distribution. Conclusion D1S80 gene loci PCR typing has good accuracy and sensitivity. It provides useful genetic information for forensic science research on individual identification and paternity test,genetic disease gene linkage analysis for D1S80 gene loci from Southern Sichuan.

关 键 词：D1S80位点 VNTR 遗传多态性 川南地区 

分 类 号：Q786[生物学—分子生物学]