人卵泡液hCG水平与卵母细胞成熟及早期胚胎发育的关系  

Correlation of follicular fluid human chorionic gonadotrophin level with oocyte maturity and early embryonic development

作　　者：张丽丽[1,2] 王海英[2] 张仁礼[2] 梁洁玲[2] 刘彩霞[2] 周艳[2] 闻安民[2] 

机构地区：[1]南方医科大学,广东广州510515 [2]广东省人民医院//广东省医学科学院生殖医学科,广东广州510080

出　　处：《南方医科大学学报》2014年第2期260-264,共5页Journal of Southern Medical University

基　　金：广东省自然科学基金(9151008004000030);广东省医学科研基金(A2012028)

摘　　要：目的：探讨控制性卵巢刺激周期中，取卵日卵泡液hCG水平与获卵数、卵母细胞成熟率、胚胎发育和妊娠结局的关系。方法分析2012~2013年间进行的311个IVF/ICSI-ET周期，化学发光法检测取卵日卵泡液hCG值，并根据hCG值进行分组，比较各组获卵数、卵母细胞成熟率、受精率、卵裂率、可利用胚胎率和临床妊娠率的差异。结果在IVF/ICSI-ET治疗周期中，取卵日hCG水平14~21 nmol/L组卵母细胞成熟率和受精率显著高于其它各组（P＆lt;0.05），获卵数、卵裂率、可利用胚胎率以及临床妊娠率与其它各组相比较高，但不存在显著差异（P＆gt;0.05）。取卵日hCG水平＆gt;21 nmol/L组卵母细胞成熟率和受精率显著低于其它各组（P＆lt;0.05），可利用胚胎率和临床妊娠率与其它各组相比较低，但不存在显著差异（P＆gt;0.05）。结论取卵日卵泡液hCG水平与卵母细胞成熟率、受精能力、胚胎发育潜能和最终的临床结局相关，取卵日卵泡液hCG水平过高可能不利于卵母细胞成熟和胚胎发育。Objective To explore the correlation of human chorionic gonadotrophin （hCG） level in the follicular fluid on oocyte retrieval day with the number of oocytes retrieved, maturation rate, embryonic development, and pregnancy outcome in controlled ovarian stimulation cycles. Methods The data of 311 IVF/ICSI-ET cycles from 2012 to 2013 was analyzed and stratified according to hCG level in follicular fluid on oocyte retrieval day （＆lt;7 nmol/L, 7-14 nmol/L, 14-21 nmol/L, and ＆gt;21 nmol/L） determined with chemiluminesence method. The number of oocytes retrieved, oocyte maturation rate, fertilization rate, cleavage rate, available embryo rate and pregnancy rate were compared between the groups. Results In the IVF/ICSI-ET cycles, the cycles with hCG level of 14-21 nmol/L in the follicular fluid on the day of oocyte retrieval had significantly higher oocyte maturation rate and fertilization rate than those in the other 3 groups （P＆lt;0.05）, but the number of oocytes retrieved, cleavage rate, available embryo rate and pregnancy rate, though slightly higher, showed no significant difference from the other 3 groups （P＆gt;0.05）. In the group with hCG level ＆gt;21 nmol/L, the oocyte maturation rate and fertilization rate were significantly lower than those in the other 3 groups （P＆lt;0.05）, and the available embryo rate and pregnancy rate were slightly lower without significant differences from the other 3 groups （P＆gt;0.05）. Conclusion Follicular fluid hCG level on the day of oocyte retrieval is associated with oocyte maturation, fertilization, embryonic development potential, and IVF outcome. An excessively high follicular fluid hCG level on the day of oocyte retrieval may have negative effects on oocyte maturation and embryo development.

关 键 词：卵泡液 体外受精 胚胎发育 妊娠结局 

分 类 号：R711.6[医药卫生—妇产科学]