TLRs基因在牦牛雄性生殖器官中表达情况的检测  

Expression profile of TLRs gene in the reproductive organs of male Bos grunniens

作　　者：曹随忠[1] 李计尚[1] 张于[1] 廖纯颖 沈留红[1] 余树民[1] 姚学萍[1] 

机构地区：[1]四川农业大学动物医学院动物疫病与人类健康四川省重点实验室,四川雅安625014

出　　处：《中国兽医科学》2014年第2期193-198,共6页Chinese Veterinary Science

基　　金：四川省教育厅青年基金项目(09zb054);教育部"长江学者和创新团队发展计划"创新团队项目(IRT0848)

摘　　要：为了解Toll样受体（TLRs）基因在牦牛生殖系统中的表达情况，应用半定量RT—PCR技术检测了TLRs基因mRNA在雄性牦牛生殖组织中的表达水平。结果显示，TLR1mRNA和TLR3～TLR9mRNA在睾丸、附睾、输精管和阴茎中均相对高表达；TLR2mRNA在睾丸、附睾头、附睾体和阴茎中相对高表达，在附睾尾和输精管中相对低表达；TLR10mRNA在睾丸、附睾头、附睾体和阴茎中相对低表达，在附睾尾和输精管中不表达。结果表明，TLRsmRNA在雄性牦牛生殖组织中均有不同程度的表达，提示它在牦牛生殖过程中可能发挥一定的作用。The purpose of the present research was to investigate expression of Toll-like recptors （TLR） gene in the reproductive organs of male yak（Bos grunniens）. Semi-quantitation RT-PCR was used for analyzing the expression profile of the TLRs mRNA in the reproductive organs of male Yak. In result, the levels of TLR1 mRNA and TLR3--TLR9 mRNA were high in the testis, epididymis, spermatic duct and penis. TLR2 mRNA was abundant in the testis, caput and corpus epididymidis, and penis, but relatively lower in cauda epididymidis and spermaduct. The level of TLR10 mRNA was low in the testis, caput and corpus epididymidis, and penis, but not detected in the cauda epididymidis and spermaduct. The results show that TLR gene may play a role in reproduction of male yak.

关 键 词：牦牛 Toll样受体基因 组织表达 

分 类 号：S852.163[农业科学—基础兽医学]