HPLC法测定归脾丸中木香烃内酯和去氢木香内酯的含量  

Determination of costunolide and dehydrocostuslactone in Guipi Pills by HPLC

作　　者：邓俊杰 孙立恩 

机构地区：[1]浙江省绍兴市食品药品检验所,绍兴312000

出　　处：《海峡药学》2014年第1期76-78,共3页Strait Pharmaceutical Journal

摘　　要：目的 建立HPLC法测定归脾丸中木香烃内酯和去氢木香内酯的含量的方法.方法 采用HPLC法,色谱柱为Agilent Eclipse SB-C18柱（150mm×4.6mm,5μm）,流动相为乙腈：0.1%磷酸溶液（45：55）,流速为1.0mL·min-1,检测波长为225nm.结果 木香烃内酯在0.1018～1.018μg间线性良好,r=0.9999,平均回收率为99.37%,RSD（n=9）为1.2%;去氢木香内酯在0.1034～1.034μg间线性良好,r=0.9999,平均回收率为98.60%,RSD（n=9）为0.9%.结论 该方法灵敏、准确、简便、重复性好,可作为归脾丸的定量控制方法.OBJECTIVE To establish an HPLC method for the determination of costunolide and dehydrocostus- lactone in Guipi Pills. METHODS The HPLC system consisted of the Agilent Eclipse SB-Cls （ 150mm × 4.6mm, 5μ m） and aeetonitrile-0. 1% H3P04（45：55） as the mobile phase,The flow rate was 1.0mL · min-1 and the detec- tive wavelength was 225nm. RESULTS Costunlide showed a good linear relationship at the range of 0. 1018 ~ 1. 018μg, r =0. 9999 ,the average recovery was 99.37% and RSD（n = 9） was 1.2%. Dehydrocostuslactone showed a good linear relationship at the range of 0. 1034 - 1. 034μ g,r =0. 9999 ,the average recovery was 98.60% and RSD （n = 6） was 0.9%. CONCLUSION The method is sensitive, simple, accurate and repeatable. It can be used for the quality control of Guipi Pills.

关 键 词：归脾丸 木香烃内酯 去氢木香内酯 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]