玻璃酸钠联合免疫三氧对大白兔创伤性滑膜炎的影响  

Effects of Sodium Hyaluronate Combined with Oznoe on Rabbit Traumatic Synovitis

作　　者：杨敏[1] 杨钰潼 郭明阳[1] 张俊[1] 罗勇[1] 袁维莉 郭玲琳[1] 晏姣[1] 贠明东[1] 刘德芳[1] 

机构地区：[1]解放军成都军区总医院,四川成都610083 [2]成都高新安德门诊部,四川成都610000

出　　处：《风湿病与关节炎》2014年第2期21-26,共6页Rheumatism and Arthritis

基　　金：四川省中医药管理局项目(2012-E-063);全军医学科研"十五"计划面上项目(01MA142)

摘　　要：目的:观察玻璃酸钠注射液联合免疫三氧治疗创伤性滑膜炎动物模型的疗效。方法:分别用空气、免疫三氧、玻璃酸钠注射液、免疫三氧+玻璃酸钠注射液关节腔灌注治疗创伤性滑膜炎动物模型,7周后对膝关节滑膜进行免疫组化观察和病理学评分,检测核因子-κB、肿瘤坏死因子-α、基质金属蛋白酶-3、基质金属蛋白酶-9、一氧化氮和诱导型一氧化氮合酶的表达。结果:免疫三氧组、玻璃酸钠组、免疫三氧+玻璃酸钠组的病理学评分、核因子-κB、肿瘤坏死因子-α、基质金属蛋白酶-3、基质金属蛋白酶-9、一氧化氮和诱导型一氧化氮合酶的表达均显著低于空气组(P<0.05)。免疫三氧组的炎细胞浸润评分要显著优于玻璃酸钠组(P<0.05),且免疫三氧组和免疫三氧+玻璃酸钠组的一氧化氮和诱导型一氧化氮合酶的浓度显著低于玻璃酸钠组(P<0.05)。两者联合使用对滑膜增生的抑制作用要显著优于单用玻璃酸钠治疗(P<0.05),而在骨和软骨的破坏方面,联合治疗比单独使用玻璃酸钠或者免疫三氧,均能取得更显著的疗效(P<0.05)。结论:免疫三氧对创伤性滑膜炎的作用侧重于抗炎,而玻璃酸钠主要是对关节骨和软骨的保护作用,两者联用治疗创伤性滑膜炎时能获得更多的疗效。Objective：To observe the therapeutic effects of sodium hyaluronate injection combined with ozone in the treatment of traumatic synovitis in animal model.Methods：Articular cavity perfusion respectively with air,ozone,sodium hyaluronate injection,and ozone plus sodium hyaluronate injection was made in the treatment of traumatic synovitis in animal models,after 7 weeks,making the immunohistochemical observation and pathological score for the synovium of knee joints and detecting the nuclear factor-κB,tumor necrosis factor-α,matrix metalloproteinase-3,matrix metalloproteinase-9,nitric oxide and the expression of inducible nitric oxide synthase.Results：The pathological score,nuclear factor-κB,tumor necrosis factor-α,matrix metalloproteinase-3, matrix metalloproteinase-9,nitric oxide and the expression of inducible nitric oxide synthase in the groups treated respectively with ozone,sodium hyaluronate injection,and ozone plus sodium hyaluronate injection were significantly lower than those in the air group（P＆lt; 0.05）.The inflammatory cells infiltration score of sodium hyaluronate were signiifcantly lower than those of the sodium hyaluronate group.The inhibitory effect on synovium hyperplasia with combined ozone and sodium hyaluronate was better than that of sodium hyaluronate in （P＆lt;0.05）,and in the treatment of the destruction of bone and cartilage,the therapeutic effect of combined ozone and sodium hyaluronate （P＆lt;0.05） or ozone （P＆lt;0.05） was better than that of sodium hyaluronate （P＆lt; 0.05）.Conclusion：The effects of ozone on traumatic synovitis mainly focus on anti-inflammation,and those of sodium hyaluronate mainly play a protective role for articular cartilage and bone,but the combination of both has a better therapeutic effect in the treatment of traumatic synovitis.

关 键 词：创伤性滑膜炎 玻璃酸钠 免疫三氧 大白兔 

分 类 号：R686.7[医药卫生—骨科学]