西黄丸挥发油抗肿瘤作用及其免疫学机制的实验研究  

Experimental Study on Antitumor Effect of Volatile Oil of Xihuang Pill and Its Immune Mechanism

作　　者：杨伟[1] 关硕[1] 胡俊霞[1] 曾常茜[1] 梁文波[1] 马杰[2] 高文斌[3] 

机构地区：[1] 大连大学医学院 大连 116622 [2] 遵义医学院 遵义 563000 [3] 大连大学附属中山医院 大连 116000

出　　处：《世界科学技术-中医药现代化》2014年第1期68-72,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：大连金州新区科学技术局高新技术研究开发计划(2012-A1-046):西黄丸抗肿瘤活性成分制备及其高效制剂的开发,负责人:梁文波

摘　　要：目的:研究西黄丸挥发油组分对荷瘤大鼠的抗肿瘤作用及其免疫学机制,筛选西黄丸抗肿瘤的活性部位。方法:雌性Wistar大鼠70只,随机抽取10只为空白对照组,剩余60只建立Walker256乳腺癌细胞荷瘤大鼠模型,建模后随机分为阴性对照组(模型组)、挥发油高、中、低剂量组,西黄丸高剂量组和香菇多糖组(阳性对照组),每组10只,灌胃给药,2次/天,连续14天。腹主动脉采血,取瘤组织称重,计算抑瘤率;采用ELISA法检测外周血IL-2、IL-6、IL-10、IFN-γ和TGF-β水平;采用流式细胞术检测CD3+T细胞、CD4+T细胞、CD8+T细胞、粘附分子B7-1(CD80)细胞含量。结果:挥发油高、中剂量组抑瘤率分别为28.4%、24.1%;与模型组比较,挥发油高、中剂量组IL-2和IFN-γ平均水平及CD3+T细胞、CD8+T细胞、B7-1含量均明显升高(P<0.05)。结论:西黄丸挥发油具有一定的抗肿瘤作用,是西黄丸抗肿瘤活性部位之一;西黄丸挥发油可通过上调外周血IL-2、IFN-γ水平及CD3+T细胞、CD8+T细胞、B7-1细胞含量提高荷瘤大鼠免疫清除功能。This study was aimed to explore the antitumor effect of volatile oil of X ihuang pill and its immune mecha-nism in order to screen the antitumor active site of Xihuang pill. Among 70 female Wistar rats, 10 rats were random-ly selected as the blank control group; and the other 60 rats was used in the establishment of walker 256 breast can-cer cell tumor-bearing rat models. The model rats were randomly divided into the negative control group （model group）, high-dose volatile oil group, middle-dose volatile oil group, low-dose volatile oil group, high-dose Xihuang pill group, and lentinan group （positive control group）, with 10 rats in each group. The intragastric administration was given twice a day for 14 days. Blood was taken from the abdominal aorta. Tumor tissues was removed and weighed to calculate the tumor inhibitory rate. ELISA method was used to detect the level of IL-2, IL-6, IL-10, IFN-γ and TGF-β in peripheral blood. The flow cytometry was used to detect the content of CD3＋ T cell, CD4＋ T cell, CD8＋ T cell, and B7-1 cell （CD80）. The results showed that the tumor inhibitory rates of volatile oil of high-dose group and middle-dose group were 28.4% and 24.1%, respectively. Compared with the model group, the average level of IL-2 and IFN-γ of volatile oil of high-dose group and middle-dose group and CD3＋ T cell, CD8＋ T cell, B7-1 cell con-tent were obviously increased （P〉.05）. It was concluded that volatile oil of Xihuang pill had certain antitumor ef-fect, which was one of the antitumor active sites of Xihuang pill. The volatile oil of Xihuang pill upregulates the lev-els of IL-2 and IFN-γ, as well as the contents of CD3＋ T cell, CD8＋ T cell, B7-1 cell in order to increase the im-mune clearance function of tumor-bearing rats.

关 键 词：西黄丸挥发油 Walker256乳腺癌细胞 大鼠 细胞因子 T细胞亚群 B7-1 免疫清除 

分 类 号：R285.5[医药卫生—中药学]