滋补脾阴方药对脾阴虚糖尿病大鼠下丘脑胰岛素信号的调控作用  

Regulation of Insulin Signaling in the Hypothalamus of Spleen Yin Deficiency Diabetes Rats Treated with Zibu Piyin Recipe

作　　者：梁丽娜[1] 战丽彬[2] 郑路平[1] 胡守玉[2] 闫云[3] 隋华[1] 张福良[4] 

机构地区：[1] 大连医科大学中西医结合学院 研究院 大连 116044 [2] 大连医科大学附属二院 大连 116023 [3] 南阳市第一人民医院 南阳 473002 [4] 大连医科大学公共卫生学院 大连 116044

出　　处：《世界科学技术-中医药现代化》2014年第1期82-86,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委面上项目(30772847):糖尿病脑病的内质网应激机制及滋补脾阴方药干预研究,负责人:战丽彬;教育部高等学校博士学科点专项科研基金(20070161004):脾阴虚痴呆与糖尿病脑病的内质网应激机制研究,负责人:战丽彬;辽宁省教育厅高校优秀人才支持计划(LR201015):滋补脾阴方药对糖尿病脑病内质网应激机制的研究,负责人:战丽彬;辽宁特聘教授资助计划,负责人:战丽彬

摘　　要：目的:通过观察胰岛素信号通路中关键分子的变化,进一步探讨脾阴虚糖尿病认知功能障碍的发病机制及滋补脾阴方药的作用机制,以期为糖尿病认知功能障碍的治疗提供新思路和新线索。方法:将大鼠随机分为空白对照组(Cont),糖尿病组(DM),脾阴虚组(pi),脾阴虚糖尿病组(piDM),滋补脾阴方药治疗组(ZBPYR)5组。采用Western blotting的方法观察下丘脑中胰岛素受体底物1(IRS-1)丝氨酸磷酸化水平及蛋白激酶C(PKC/Akt)丝氨酸磷酸化水平,以判定胰岛素信号传导是否出现障碍。结果:DM组、pi组、piDM组p-IRS-1ser表达较Cont组增加(P<0.05),DM组、piDM组p-Akt表达减弱(P<0.05)。ZBPYR组p-IRS-1ser较DM组、piDM组减弱(P<0.05),p-Akt表达较DM组、piDM组增加(P<0.05)。结论:DM组、pi组、piDM组大鼠下丘脑中胰岛素信号未正常向下传导,可能发生了胰岛素信号转导障碍。ZBPYR具有纠正胰岛素信号转导障碍的作用。This study was aimed to observe changes of key molecular in insulin signaling pathway in the hypothala-mus of rats to explore the mechanism of spleen yin deficiency diabetes-associated cognitive decline （DACD） and Zibu Piyin Recipe （ZBPYR） in order to provide new ideas and new clues for treatment of DACD. Rats were randomly divided into five groups, which were the control （Cont） group, diabetes （DM） group, spleen yin deficiency （pi） group, spleen yin deficiency diabetes （piDM） group and the ZBPYR group. Insulin receptor substrate 1 （IRS-1） serine phos-phorylation levels and protein kinase B （PKB/Akt） serine phosphorylation levels which were involved in the insulin signaling were observed by western blotting in the hypothalamus to determine whether there were insulin signaling obstacles in the hypothalamus of rats. The results showed that the expression of p-IRS-1ser in the DM group, pi group and piDM group was increased compared with the Cont group （P〉.05）; while the p-Akt expression of the DM group and piDM group was decreased （P〉.05）. The expression of p-IRS-1ser in the ZBPYR group decreased＆amp;nbsp;compared with the DM group and piDM group （P〉.05）; while the level of p-Akt increased compared with the DM group and piDM group （P 〉.05）. It was concluded that insulin signaling was not transduced normally in the hy-pothalamus of the DM group, pi group and piDM group. Insulin resistance may occur in the hypothalamus. And ZBPYR can correct insulin signaling transduction disorder.

关 键 词：脾阴虚糖尿病 认知功能障碍 脑胰岛素抵抗 滋补脾阴方药 

分 类 号：R285.5[医药卫生—中药学]