鼻渊舒口服液对慢性鼻-鼻窦炎模型鼻窦黏膜上皮GR及IκBα表达的影响  

Influence on Nasal Sinuses Mucosa Epithelium GR and IκBα Expression for Chronic Rhinosinusitis Model Treated with Bi-Yuan-Shu Oral Liquid

作　　者：李辉[1] 朱天民[2] 

机构地区：[1] 成都大学医护学院 成都 610106 [2] 成都中医药大学针灸推拿学院 成都 610075

出　　处：《世界科学技术-中医药现代化》2014年第1期98-102,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然基金委青年项目(81102621):基于分子伴侣HSP70/CHIP与NF-κB炎症平衡体系的清热益气通窍法干预CRS上皮炎症的研究,负责人:李辉;国家自然科学基金委青年项目(30801480):清热益气通窍法干预CRS上皮炎症研究,负责人:朱天民

摘　　要：观察鼻渊舒口服液对慢性鼻-鼻窦炎(CRS)模型鼻窦黏膜上皮糖皮质激素受体(GR)及核因子κB抑制蛋白(IκBα)表达的影响,从抑炎机制角度,探索鼻渊舒对CRS的治疗机制。方法:选取新西兰大白兔100只,按每组20只,随机分为正常组、假手术组、模型组、鼻渊舒组、克拉霉素组后,建立CRS模型,正常组、假手术组、模型组不干预,鼻渊舒、克拉霉素组分别给予鼻渊舒(1.5 mL·kg-1·d-1)、克拉霉素(25 mg·kg-1·d-1)灌胃14天,治疗结束后取鼻窦黏膜,HE染色观察其病理改变,Western Blotting法检测鼻窦黏膜上皮细胞胞浆糖皮质激素受体(GR)及核因子κB抑制蛋白(IκBα)蛋白表达。结果:模型组鼻窦黏膜炎细胞明显浸润,呈慢性炎症病变,腺体及杯状细胞明显增生;与正常组比较,GR表达显著降低(P<0.01),IκBα表达显著增高(P<0.01)。鼻渊舒灌胃治疗后,鼻窦黏膜上皮得到较好修复,炎细胞浸润不明显,腺体和杯状细胞增生亦不明显;与模型组比较,GR表达显著增高(P<0.01),IκBα表达显著降低(P<0.01)。结论:鼻渊舒在促进抑炎途径的GR表达的同时,通过抑制IκBα表达,防止IκBα对NF-κB促炎途径的过度抑制,动态调控了鼻窦黏膜上皮炎症的平衡。This study was aimed to investigate the influence of Bi-Y uan-Shu （BYS） Oral Liquid on glucocorticoid re-ceptor （GR） and nuclear factor IκBα expression of nasal sinuses mucosa epithelium among chronic rhinosinusitis （CRS） models in order to explore its therapeutic mechanism for CRS from the anti-inflammatory reaction aspect. One hundred New Zealand rabbits were selected and randomly divided into the normal group, sham operation group, model group, BYS group, and clarithromycin group, with 20 rabbits in each group. After the CRS model was established, no intervention was given to the normal group, sham operation group, or model group. The intragastric administrations of BYS （1.5 mL＆#183;kg-1＆#183;d-1） and clarithromycin （25 mg＆#183;kg-1＆#183;d-1） were given for 14 days, respectively. The nasal sinuses mucosa was taken after the treatment. And HE stain was used to observe its pathological changes. Western Blotting was used in the detection of nasal sinuses mucosal epithelium cytoplasm GR and IκBα expression. The results showed that there were obvious nasal sinuses mucosa inflammatory cell infiltration, chronic inflammation changes, and obvious hyperplasia of glandular organs and goblet cells. Compared with the normal group, the GR expression was obviously reduced （P〉.01）. And the IκBα expression was obviously increased （P〉.01）. After the intragastric administration of BYS, the nasal sinuses mucosal epithelium was repaired with no obvious inflammatory cell infiltration, glandular organs, or goblet cells. Compared with the model group, the GR expression was obviously increased （P〉.01）. The IκBα expression was obviously decreased （P〈0.01）. It was concluded that BYS can promote GR expression to inhibit inflammation. Meanwhile, it can restrain IκBα expression to prevent the over inhabitation of IκBα on NF-κB. It can dynamically regulate the balance of the nasal sinuses mucosa epithelium inflammation.

关 键 词：鼻渊舒 慢性鼻原鼻窦炎 鼻窦黏膜上皮 糖皮质激素受体 IΚBΑ IΚBΑ 

分 类 号：R285.5[医药卫生—中药学]