西藏沙棘质量标准研究  

Research on Quality Standards of Fruit of Hippophae Tibetana Schlechtend.

作　　者：杨洋[1] 苏永文 刘悦[1] 游佳莉[1] 张艺[1] 王毓杰[1] 

机构地区：[1]成都中医药大学民族医药学院,成都611137

出　　处：《世界科学技术-中医药现代化》2014年第1期146-150,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：四川省科学技术厅科技支撑计划项目(2013SZ0114):特色中藏药资源利用的关键技术及产品开发研究;负责人:张艺;四川省教育厅省属高校科研创新团队建设计划(11TD004):中藏药资源保护与利用;负责人:张艺;四川省药检所藏药材标准(2013年版)科研任务:西藏沙棘质量标准研究;负责人:张艺

摘　　要：目的:建立西藏沙棘药材质量标准。方法:采用显微法及TLC法鉴别西藏沙棘药材;按照2010年版《中国药典》(一部)测定西藏沙棘药材水分、总灰分、酸不溶性灰分及醇溶性浸出物含量;采用HPLC法测定其槲皮素、山柰素及异鼠李素含量。结果:确定西藏沙棘药材显微特征,建立槲皮素、山柰素及异鼠李素3种黄酮类成分的薄层鉴别及含量测定方法。结论:本研究建立的定性、定量方法,可用于西藏沙棘药材质量控制。This study was aimed to establish quality standards of fruit of Hippophae tibetana Schlechtend.. The medical material was identified by microscopy and thin layer chromatography （TLC）. According to methods recorded in the Chinese Pharmacopoeia （2010 edition）, the contents of moisture, total ash, acid-insoluble ash and alcohol-sol-uble extract were determined. The HPLC method was applied in the determination of quercetin, kaempferol and isorhamnetin content. The results showed that the microscopic characteristics of Tibetan medicine H. tibetana were identified. The TLC and content determination methods of quercetin, kaempferol and isorhamnetin were estab-lished. It was concluded that the established qualitative and quantitative methods can be used for quality control of the fruit of H . tib e tana.

关 键 词：藏药 西藏沙棘 质量标准 显微鉴别 薄层鉴别 含量测定 

分 类 号：R291.4[医药卫生—民族医学]