正交试验法优化清开灵注射液中金银花提取液的醇沉工艺  

Optimization of Alcohol Precipitation Techniques for Honeysuckle Extract of Qingkailing Injection by Orthogonal Test

作　　者：孙秀玉[1] 王英姿[1] 乔延江[1] 冯艾灵[1] 段飞鹏[1] 周海燕 

机构地区：[1] 北京中医药大学中药学院 北京 100102 [2] 亚宝北中大制药有限公司 北京 101300

出　　处：《世界科学技术-中医药现代化》2014年第1期187-192,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：科学技术部国家“重大新药创制”项目(2010ZX09502-002):清开灵注射液安全性关键技术研究,负责人:乔延江;北京中医药大学创新团队(2011-CXTD-13):复方中药制药研究,负责人:杜守颖

摘　　要：目的:优化金银花提取液的醇沉工艺,规范清开灵注射液中金银花提取液的生产,减少批次间差异。方法:采用正交试验,以绿原酸和木犀草苷的含量为指标,并采用多指标综合评分法处理数据,优化醇沉工艺中加醇时的药液温度、搅拌速度和加醇速度3个因素。结果:金银花提取液的最佳醇沉工艺为加醇时药液温度为20℃,搅拌速度240 rpm,加醇速度1倍生药量/min。结论:优化的醇沉工艺稳定可行。This study was aimed to optimize the alcohol precipitation techniques for honeysuckle extract in order to standardize the production of honeysuckle extract of Qingkailing Injection and reduce the differences among batches. The orthogonal test was applied in this study. The content of chlorogenic acid and galuteolin were taken as compre-hensive indicators. Multi-index comprehensive scoring method was used in the data analysis. Three influencing fac-tors, which were the fluid temperature, the stirring speed and the speed of adding alcohol, were optimized in the al-cohol precipitation techniques. The results showed that the optimal alcohol precipitation techniques were when the fluid temperature was 20℃, the stirring speed was 240 rpm and the speed of adding alcohol was one time of the ma-terial per minute. It was concluded that the optimized alcohol precipitation process was stable and feasible.

关 键 词：清开灵注射液 金银花 正交试验 醇沉工艺 绿原酸 木犀草苷 

分 类 号：TQ460.6[化学工程—制药化工]