麦冬酒制前后对内皮细胞缺氧保护作用的比较研究  

Comparative study of protective effect of Radix Ophiopogonis processed Protective effect of Radix Ophiopogonis on hypoxia before and after being processed by wine-a comparative study

作　　者：焦艳[1] 李喆[1] 刘清新[1] 于永军[1] 

机构地区：[1]河北省沧州市沧州医学高等专科学校药学系,061001

出　　处：《环球中医药》2014年第2期110-112,共3页Global Traditional Chinese Medicine

基　　金：沧州市科学技术研究与发展指导计划(1213145ZD)

摘　　要：目的研究中药麦冬及其酒制炮制品对人脐静脉内皮细胞(EAHY926)缺氧损伤的保护作用。方法体外培养EAHY926,采用通入缺氧气体建立缺氧损伤模型。用麦冬提取液干预后,采用MTT比色法测定细胞活力、黄嘌呤氧化酶法测定细胞内超氧化物歧化酶活性、硫代巴比妥酸显色法测定丙二醛含量、硝酸还原酶法测定一氧化氮含量。结果组间比较采用单因素方差分析,两两之间用SNK检验。与模型组相比,川麦冬生品提取液组、酒制川麦冬提取液组、杭麦冬生品提取液组、酒制杭麦冬提取液组均能显著提高细胞活力(P<0.05),并且均显著提高缺氧后细胞超氧化物歧化酶活性(P<0.05)及降低丙二醛、一氧化氮的含量(P<0.05);酒制川麦冬提取液组和酒制杭麦冬提取液组效果最佳,与空白对照组比有非常显著性差异(P<0.01)。结论麦冬酒制后更能显著拮抗缺氧对EAHY926的损伤。Objective To study the Chinese medicine Radix Ophiopogonis and the protective role of its wine-processed products on hypoxia of human umbilical vein endothelial cells（ EAHY926）. Methods In vitro culture of EAHY926 was performed,which was exposed to hypoxic gas to establish models with hypoic injury. After intervention by Radix extract,MTT colorimetry was used to determine cell viability,the xanthine oxidase method was adopted to detect the activity of superoxide dismutases（ SOD） in cells,the thibabituric acid developing method was adopted to assay malondialdehyde（ MDA）,and the nitrate reductase method was used to detect the content of nitric oxide（ NO）. Results Data collected was treated with one-way ANOVA,SNK test to make pairwise comparison. Compared with the model group, Ophiopogon japonicus extract raw chemicals group,wine extract Ophiopogon group,Hang Radix Ophiopogonis extract raw chemicals group,wine Hangzhou Radix Ophiopogonis extract group could significantly improve cell viability（ P ＆lt; 0. 05）,with respect to SOD activity during cell hypoxia（ P ＆lt; 0. 05） and decrease MDA and NO levels（ P ＆lt; 0. 05）; wine extract Ophiopogon group and wine Hangzhou Radix Ophiopogonis extract group showed the sharpest increase among the groups,as exhibited significant difference compared with control group（ P ＆lt; 0. 01）. Conclusion Wine-processed Radix Ophiopogonis was significantly effective in antagonizing the damage on EAHY926 caused by hypoxia.

关 键 词：麦冬 酒制 内皮细胞 缺氧保护 

分 类 号：R284[医药卫生—中药学]