石榴皮原花青素的纯化工艺及其抗李斯特菌活性研究  

Research for the purification conditions of proanthocyanidins from pomegranate peels and its antibacterial activity to Listeria monocytogenes

作　　者：柯春林[1] 孙兰萍[1] 张斌[1] 曾卫国[1] 吕超田[1] 程立胜 

机构地区：[1]蚌埠学院生物与食品工程系,安徽蚌埠233030

出　　处：《应用化工》2014年第2期261-264,共4页Applied Chemical Industry

基　　金：安徽省高等学校省级自然科学基金项目(KJ2012B094);蚌埠学院博士科研启动基金项目;蚌埠学院优秀人才基金项目(院字[2009]107号)

摘　　要：从6种大孔树脂中筛选出对石榴皮原花青素纯化效果较好的HPD-600树脂,探索该树脂对原花青素的纯化工艺。结果表明,该树脂纯化原花青素的最佳条件是:静态平衡时间8 h,提取液pH值为5.0,吸附液料比为12∶1,静置吸附时间90 min,洗脱液为80%的乙醇溶液,洗脱液料比25∶1。原花青素提取液对单增李斯特菌有抑菌作用。In order to purified the proanthocyanidins from pomegranate peels, selected six kinds of macroporous resins in this experiment and found that the HPD-600 macroporous resin showed more better separation performance to the proanthocyanidins. Result showed that the optimum conditions were as follows： static equilibrium time 8 h, extract pH value 5.0,adsorption liquid to solid ratio 12：1 ,static adsorption time 90 rain, eluting with 80% the ethanol solution, eluent liquid to solid ratio 25 ： 1. The proanthocyanidins from pomegranate peels showed moderate antibacterial activity to the Listeria monocytogenes.

关 键 词：石榴皮 原花青素 纯化工艺 李斯特菌 抗菌活性 

分 类 号：TQ281.1[化学工程—有机化工]