督脉配穴对染铅幼年大鼠海马一氧化氮合酶阳性细胞的保护作用  

Protective effects of governor meridian acupuncture on the nitric oxide synthase positive cells in young lead-poisoned rat hippocampus

作　　者：李硕[1] 张同园[1] 李积胜[1] 王晶[1] 

机构地区：[1]天津中医药大学,天津300192

出　　处：《中医儿科杂志》2014年第1期19-21,共3页Journal of Pediatrics of Traditional Chinese Medicine

基　　金：天津中医药大学科技创新基金项目(TZB2011-1)

摘　　要：目的探讨督脉配穴对染铅幼年大鼠海马不同亚区一氧化氮合酶(NOS)变化的拮抗作用。方法将40只SD大鼠随机分为3组,即对照组、染铅组、督脉配穴组。采用反映学习记忆功能的Y迷宫法测试大鼠神经行为的改变,用NADPH-黄递酶(NADPH-d)组织化学法研究大鼠海马不同亚区NOS的活性神经元变化。结果染铅组大鼠的学习记忆能力比督脉配穴组和对照组明显下降(P<0.05),但督脉配穴组与对照组之间差异无统计意义(P>0.05);组织化学实验结果显示,染铅组大鼠海马CA1区和齿状回的NOS阳性神经元明显少于督脉配穴组和对照组(P<0.05),在CA3区无差别,督脉配穴组与对照组各亚区之间差异无统计意义(P>0.05)。结论铅可损伤幼年大鼠学习记忆能力,其机制可能与染铅后海马各区NOS活性的不同变化有关,督脉配穴针灸治疗对铅引起的学习记忆损伤和NOS活性的变化有拮抗作用。Objective To investigate the antagonistic effect of governor meridian acupuncture on the nitric ox- ide synthase level in young lead-exposed rat hippocampus subarea. Methods 40 SD rats were randomly divided into three groups, normal group, lead-exposed model group and governor meridian acupuncture group. Y-maze test was applied to study the effects of lead on learning and memory ability in rats; NADPH-d histochemistry was used to investigate the changes of nitric oxide synthase （NOS） in different hippocampal subareas in lead-exposed young rat. Results Compared with the control and governor meridian acupuncture group, the learning and memory ability of lead-exposed rats was significantly decreased （P〈0.05）, but there was no statistic significance between governor meridian acupuncture group and control group （P〉0.05） ; The number of NOS positive neurons on CA1 and dentate gyrus of lead-exposed young rats were significantly decreased （ P〈0.05 ）, but no significant changes in CA3 ; No difference between the control and governor meridian acupuncture groups in CA1, CA3 and dentate gyrus （ P〉0.05 ）. Conclusion Lead may damage the learning-memory ability of the young rats, which might be related to the changes of NOS in different subfields of hippocampus in lead-exposed rats, governor meridian acupuncture might play an important role in preventing lead-inducted damages.

关 键 词：铅 一氧化氮合酶 督脉配穴 海马 

分 类 号：R245.3[医药卫生—针灸推拿学]