刺五加细胞色素P450基因的克隆与表达分析  

Cloning and Expression Analysis of Cytochrome P450 Gene in Eleutherococcus senticosus

作　　者：邢朝斌[1] 吴鹏[1] 李非非[1] 刘岩[1] 周秘[1] 修乐山[1] 

机构地区：[1]河北联合大学生命科学学院,唐山063000

出　　处：《生物技术通报》2014年第1期112-115,共4页Biotechnology Bulletin

基　　金：国家自然科学基金项目(30701086);河北省自然科学基金项目(C2009001252);河北省自然科学基金-石药集团医药联合研究基金项目(H2012401006);河北联合大学培育基金项目(GP201306)

摘　　要：根据已报道的人参、三七等植物的细胞色素P450(Cytochrome P450,P450)基因的cDNA序列设计引物,利用RT-PCR法克隆刺五加P450基因的cDNA全长序列,并分析其在不同生长发育时期和器官中的表达情况。结果显示,克隆了全长为1 410 bp的刺五加P450基因的cDNA序列,该基因编码469个氨基酸残基组成的蛋白质。GenBank登录号为KF498590,与人参、三七的P450氨基酸序列一致性分别为91.5%和90.4%。刺五加的P450基因在不同生长发育时期和器官中均有表达,但表达量具有显著差异(P<0.05)。最大表达量出现在盛花期,为最低表达量(萌芽期)的1.26倍。各器官中,叶片的表达量最高,是最低量幼茎的1.49倍。In order to clone cytochrome P450 （ P450 ） in Eleutherococcus senticosus, the primers were designed according to cDNA of the reported Panax giseng and P. notoginseng. The full length cDNA of the E. senticosus P450 was chined by RT-PCR, and analyzed the expression of P450 in different growth periods and organs of E. senticosus. The results showed that the full length of P450 was 1 410 bp, encoded a prolein with 469 amino-acid residues, GenBank accession number KF498590. To compare the amino acid sequence of E.senticosus P450 with P. ginseng and P. notoginseng, the amino acid homology was 91.5% and 90.4%. P450 expressed in different growth periods and organs of E. senticosus, and the expression amount differed significantly （ P〈0.05 ） . The highest content of the expression showed up when full opening flower stage, which was 1.26 times as much as that in the lowest at germination stage. The highest content of the expression was in the leaves which was 1.49 times as much as that of the lowest in young stem.

关 键 词：刺五加 细胞色素P450 克隆 表达分析 

分 类 号：S567.19[农业科学—中草药栽培]