液-质联用法测定妇宁丸中盐酸水苏碱的含量  

Determination of stachydrine hydrochloride in Funing pills by LC- MS / MS

作　　者：赵勇 胡娟妮 丁野 郑金凤 李文莉 

机构地区：[1]湖南省食品药品检验研究院,长沙410001 [2]中南大学药学院,长沙410013

出　　处：《药物分析杂志》2014年第2期345-348,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的：建立妇宁丸中盐酸水苏碱的含量测定方法。方法：采用LC-MS/MS法测定盐酸水苏碱的含量。使用Venusil HILIC色谱柱（100 mm×2.1 mm，3 μm），以乙腈-水（75：25）为流动相，流速0.3 mL·min^-1；以电喷雾离子源（ESI）正离子模式将样品离子化，多反应监测（MRM）模式下对盐酸水苏碱（m/z 144.0→57.91和m/z 144.0→83.96）进行测定。结果：盐酸水苏碱浓度在5.0440-201.76 ng·mL^-1线性关系良好（r=0.9999），平均回收率（n=6）为99.6%，RSD为1.3%。结论：该方法简便、可靠、准确，可作为妇宁丸中盐酸水苏碱的含量测定方法。Objective： To establish the quantitative method of determining the contents of stachydrine hydrochloride in Funing pills. Methods： The content of stachydrine hydrochloride in Funing pills was determined by LC-MS/MS.The chromatographic column was Venusil HILIC（100 mm×2.1 mm,3 μm）.The mobile phase was acetonitrile-water （75：25）,at the flow rate of 0.3 mL·min^-1.The eletrospray ionization（ESI） with positive source of the mass spectrometer and detected in multiple reaction monitor（MRM） mode was used to detect stachydrine hydrochloride（m/z 144.0→57.91 and m/z 144.0→83.96）. Results： The linear range of stachydrine hydrochloride was 5.0-201.8 ng·mL^-1（r=0.9999）.The average recovery rate（n=6） was 99.6%,and RSD was 1.3%. Conclusion： This method is simple,accurate and can be used to determine the content of stachydrine hydrochloride in Funing pills.

关 键 词：中成药 妇宁丸 盐酸水苏碱 液-质联用法 多反应监测模式 含量测定 质量标准提高 

分 类 号：R917[医药卫生—药物分析学]