猪源产志贺毒素大肠埃希菌耐药性及超广谱β内酰胺酶基因型分析  

Analysis of antimicrobial resistances and ESBLs genotypes of Shiga toxin-producing Escherichia coli isolates from pigs

作　　者：孟琼[1,2] 赵爱兰[1] 白向宁[1] 李娟[1] 熊衍文[1] 

机构地区：[1]中国疾病预防控制中心传染病预防控制所、传染病预防控制国家重点实验室,北京102206 [2]安徽省宿州市疾病预防控制中心,安徽宿州234000

出　　处：《中国病原生物学杂志》2014年第1期23-26,36,共5页Journal of Pathogen Biology

基　　金：国家“艾滋病和病毒性肝炎等重大传染病防治”科技重大专项(No.2011ZX10004-001)

摘　　要：目的了解我国猪源产志贺毒素大肠埃希菌(Shiga toxin-producing Escherichia coli,STEC)产超广谱β内酰胺酶(Extended-spectrumβ-lactamases,ESBLs)情况及其基因型。方法对22株分离自重庆地区2个规模化养殖场健康猪粪的STEC菌株进行药物敏感性检测、ESBLs表型确证及7种类型(blaSHV,blaLEN,blaOKP,blaTEM,blaCTX-M,blaGES及blaKPC)ESBLs基因型PCR检测和测序分析。结果 22株携带stx2e的STEC菌株中2株对所有23种测试抗生素敏感,其余菌株除对亚胺培南和美罗培南敏感外,对其他抗生素均存在不同程度耐药。10株STEC菌株(占45.45%)产ESBLs酶。PCR检测其中1株为blaCTX-M-1基因型,其余9株为blaCTX-M-9+blaTEM-1基因型。结论重庆地区猪源STEC中存在较高比例的产ESBLs菌株。在加强人和动物STEC感染检测与监测的同时,应进行细菌耐药性监测。Objective To ascertain the prevalence and genotypes of extended-spectrum β-1actamases （ESBLs） produced by Shiga toxin-producing Escherichia coli （STEC） strains isolated from healthy pigs in China. Methods Twenty-two STEC strains were isolated from pig feces from 2 large pig farms. A antimicrobial resistance assay and ESBL test were performed, ESBLs were genotyped using 7 genes （blaSHV, blaLEN , blaOKP, blaTEM, blaCTX- M, blaG Es, and blaKPC）, and DNA sequencing was performed. Results Two of the 22 STEC isolates that carried stx2e were susceptible to 23 antimi- crobials. Other STEC isolates had different patterns of resistance except to imipenem and meropenem. Ten STEC isolates were ESBL-positive （45. 450/00）; 1 was the blaCTX-M-1 genotype while the rest 9 were the blaCTC-M-＋blaTEM-1 genotype. Conclusion A high proportion of STEC isolates in Chongqing are ESBL-producing strains. Antimicrobial resistance should be monitored while testing for STEC infections in human and animals.

关 键 词：产志贺毒素大肠埃希菌 超广谱G内酰胺酶 基因型 

分 类 号：R378.21[医药卫生—病原生物学]