SPRN0/0 型小鼠与C57BL／6野生型小鼠小脑中microRNA的差异表达  

Differential Expression of microRNAs in Cerebellum of SPRN^(0/0) Mice and C57BL/6 Wild Mice

作　　者：白换力[1,2] 张衡[1,3] 鞠传静[1,4] 孟轲音[1] 李忠义[1] 张晓莉[1,5] 张腾龙[1,3] 薄乐[1,3] 王雄[3] 陈志宝[3] 万家余[1] 申海清[2] 

机构地区：[1]军事医学科学院军事兽医研究所,吉林长春130122 [2]吉林大学动物医学学院,吉林长春130062 [3]黑龙江八一农垦大学生命科学技术学院,黑龙江大庆163000 [4]吉林大学第四医院,吉林长春130011 [5]长春理工大学生命科学技术学院,吉林长春130022

出　　处：《中国畜牧兽医》2014年第2期16-20,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然基金(31072148、31101782、31201714、31201937);吉林省科技发展计划项目(201215096)

摘　　要：本试验通过实时荧光定量PCR方法来验证SPRN0/0型小鼠和C57BL/6野生型小鼠小脑中小分子RNA(microRNA)差异性表达,探讨SPRN基因敲除后小鼠小脑中microRNA相对表达变化。结果表明,与正常标本比,SPRN0/0型小鼠小脑中miR-135a、miR-24-2*、miR-146a、miR-7a、miR-380、miR-448、miR-128和miR-152的表达量均显著下调,其中miR-24-2*下调10倍以上,miR-448、miR-146a、miR-128、miR-380、miR-152均下调2倍以上,miR-135a和miR-7a均下调1倍。由此推断,microRNA的这些变化可能与Shadoo蛋白的生理功能有关,且microRNA靶基因的确立也可以为进一步研究Shadoo蛋白的生理功能提供新的思路。The purpose of this study was to identify different expression of microRNAs between cerebellum of SPRN0/0mice and C57BL/6 wild mice, and investigate relative expression results of mieroRNA in cerebrum of SPRN0/0 mice by Real-time PCR technology. The results showed that compared with cerebellum of C57BL/6 wild mice, eight microRNAs were decreased significantly：miR-135a, miR-24-2 ＊ , miR-146a, miR-Ta, miR-380, miR-448, miR-128 and miR-152, miR-24-2 ＊ fold change to more than 10, five microRNAs including miR-448, miR-146a,miR-128, miR-380 and miR-152 fold change to more than 2, miR-135a and miR-Ta fold change to more than 1. Thus, we concluded that these changes of mieroRNAs expression might be involved in Shadoo protein function and the establishment of target genes of microRNAs might provide new ideas for researc hing Shadoo protein function.

关 键 词：Shadoo蛋白 MICRORNA 实时荧光定量PCR 小脑 

分 类 号：Q786[生物学—分子生物学]