抗生长激素受体单克隆抗体的功能特点  

Functional Characterization of Anti-growth Hormone Receptor Monoclonal Antibody

作　　者：杨艳红[1] 付志玲[1] 刘禹[1] 兰海楠[1] 张辉[1] 吴旻[1] 马思慧[1] 李雨萌[1] 李雁强[1] 郑鑫[1] 

机构地区：[1]吉林农业大学动物科技学院,吉林长春130118

出　　处：《中国畜牧兽医》2014年第2期25-28,共4页China Animal Husbandry & Veterinary Medicine

基　　金："十二五"农村领域国家科技计划(2013BAD10B03);吉林省教育厅十二五技术研究项目(201239)

摘　　要：生长激素受体(growth hormone receptor,GHR)属于Ⅰ类细胞因子受体,是调节机体后天生长的主要因子,并介导了生长激素(growth hormone,GH)所调节的多种代谢与合成功能。GHR单克隆抗体(monoclonal antibody,mAb)一直是研究这种受体的一种很好的工具,对1H2、1A9、2C3和mAb263这4种抗GHR mAb进行分析描述可知,1A9可以与受体结合,但是不能促进垂体切除鼠增长;2C3可以促进垂体切除鼠生长,然而这种作用会在GH存在的情况下受到抑制;mAb263作为一种受体激活剂抗体,不仅能与受体结合,同时还能够诱导受体发生与GH刺激相近但是不一致的受体构象变化,起到模拟GH的作用。The growth hormone receptor （GHR） is the key regulator of postnatal growth which belongs to the type Ⅰ cyto- kine receptor that mediates many functions regulated by growth hormone （GH）. Monoclonal antibodies （mAb） to the GHR have been important tools for the study of this receptor. All these anti-GHR mAbs, designated 1H2, 1A9, 2C3 and mAb263, are highly reactive with GHR, but 1A9 does not promote growth in hypophysectomized rats, 2C3 can do and this effect will be inhibited in the presence of GH. mAb263 as the commercial monoclonal antibody that binding to the receptor has a similar effect with GH, not only can recognise GHR,but also can induce a conformational change of the receptor in a similar manner, but not identical with GH.

关 键 词：生长激素受体 生长激素 单克隆抗体 亚域1 

分 类 号：Q575.11[生物学—生物化学]