大观霉素人工抗原的合成及其多克隆抗体制备  

Synthesis of Artificial Antigen of Spectinomycin and Preparation of its Polyclonal Antibody

作　　者：王照鹏 李向梅[1,2] 徐飞[2] 王文珺 苏丽芳 温凯[2] 吴小平 

机构地区：[1]北京维德维康生物技术有限公司,北京100095 [2]中国农业大学动物医学院,北京100193

出　　处：《中国畜牧兽医》2014年第2期54-57,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家十二五科技支撑计划项目(2011BAK10B01-04)

摘　　要：以大观霉素(SPE)、盐酸羧甲基羟胺(CMO)、乙二醇二环氧甘油醚(EDE)、牛血清白蛋白(BSA)、鸡卵清白蛋白(OVA)等为原料,采用活泼酯法和EDE双功能团活泼试剂方法分别合成2种免疫原和2种包被原。以免疫原免疫家兔制备多克隆抗体,以建立的间接ELISA方法来测定多克隆抗体效价及灵敏度。结果表明,免疫原SPE-CMO-BSA具有良好的免疫原性,制备的多克隆抗体经ELISA检测效价可达1∶160000,IC50为0.484ng/mL。本研究结果为下一步SPE残留快速检测试剂盒的研制奠定了基础。Using spectinomycin （SPE）, carboxyl methyl hydroxylamine hydrochloride （CMO）, glycol diglyeidyl ether （EDE）, bovine serum albumin （BSA） and ovalbumin （OVA） as the main stuff, four artificial-immunogens had been obtained by the N-hydroxysuccinimide-activated ester method and the EDE double functional group method. Ten female rabbits were im- munized with this two artificial-immunogens. Animal immune test demonstrated that there were antibodies against SPE in rab- bits sera. The polyclonal antibody was measured with indirect ELISA test. The titer was 1 ： 160000 and the IC50 was 0. 484 ng/mL. This study laid the basis for further research on the immune test kit for the SPE remnant.

关 键 词：大观霉素 免疫原 多克隆抗体 效价 

分 类 号：Q813.2[生物学—生物工程]