日粮硒对小鼠肾脏硒沉积及抗氧化酶基因表达的影响  

Effect of Dietary Selenium on Selenium Deposition and Antioxidant Enzymes Gene Expression in Mouse Kidney

作　　者：刘碧涛[1] 张琴[1] 郑良焰[1] 郭凯[1] 余文兰[1] 刘正伟[1] 郭翠丽[1] 陈叶[1] 唐兆新[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642

出　　处：《中国畜牧兽医》2014年第2期87-91,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(30871900)

摘　　要：本试验旨在研究日粮硒对小鼠肾脏硒沉积及抗氧化酶基因表达的影响。试验Ⅰ、Ⅱ、Ⅲ、Ⅳ组分别以0.045、0.1、0.4和0.8mg/kg含硒量的试验日粮连续饲喂小鼠8周。结果显示,肾脏硒沉积水平随日粮中硒含量增加而升高,Ⅰ组显著低于其他组(P<0.05),饲喂到第56天,Ⅱ、Ⅲ、Ⅳ组组间差异均不显著(P>0.05);Ⅰ组的硫氧还蛋白还原酶2(TrxR2)mRNA表达量显著低于Ⅱ、Ⅲ组(P<0.05),Ⅱ组高于Ⅲ组,且差异显著(P<0.05);Ⅰ组的超氧化物歧化酶1(SOD1)和超氧化物歧化酶2(SOD2)mRNA表达量均显著低于Ⅱ、Ⅲ组(P<0.05),Ⅱ、Ⅲ组间SOD1表达量差异不显著(P>0.05),SOD2表达量差异显著(P<0.05),且Ⅱ组高于Ⅲ组;Ⅰ、Ⅱ组的过氧化氢酶(CAT)mRNA表达量显著高于Ⅲ组(P<0.05),但Ⅰ、Ⅱ组间差异不显著(P>0.05)。结果表明,补硒可提高肾脏组织硒沉积水平,且随着饲喂时间增加,肾脏硒沉积会在高硒添加组中趋于饱和;缺硒和补硒过量都会降低肾脏TrxR2、SOD1、SOD2和CAT mRNA的表达水平。This study was to explore the effect of dietary selenium on selenium deposition and antioxidant enzymes gene ex- pression in mouse kidney. Four groups （ Ⅰ , Ⅱ , Ⅲ and Ⅳ）of mice were fed eight weeks with 0. 045,0.1,0.4 and 0.8 mg/kg Se, respectively. The results showed that the levels of kidney selenium deposition increased with the increasing of dietary sele- nium, group Ⅰ was significantly lower than other groups （P〈0.05）. Kidney selenium deposition among Ⅱ. Ⅲ. Ⅳ groups showed no significant differences when fed to the 56th day （P〈0.05）. The TrxR2 mRNA expression of group Ⅰwas signifi- cantly lower than that of groups Ⅱ and Ⅲ （P〈0.05）, and the two groups also showed significant differences（P〈0.05）. The SOD1 and SOD2 mRNA expression levels of group Ⅰ were significantly lower than that of groups Ⅱ and Ⅲ （P〈0.05）. SOD2 expression showed significantly different between groups Ⅱ andⅢ （P〈0. 05）, while SOD1 expression showed no significant differences between the two groups. The CAT mRNA expression levels of groups Ⅰ and Ⅱ were significantly higher than that of group Ⅲ （P〈0.05）, there were no significant differences between groupsⅠ and Ⅱ （P〉0.05）. These results indicated that selenium could increase the level of kidney selenium deposition. With the feeding time increased, kidney selenium deposi- tion in high-selenium group showed saturated. Se deficient and excessive could reduce the mRNA expression levels of kidney antioxidant genes （TrxR2, SOD1, SOD2 and CAT）.

关 键 词：硒 肾脏硒沉积 抗氧化酶 基因表达 

分 类 号：Q786[生物学—分子生物学]