3种抗凋亡试剂在猪精液低温保存中的应用  

Application of Three Anti-apoptotic Reagents to the Low Temperature Preservation of Boar Semen

作　　者：许春荣 陆媚[2] 刘德玉 唐胤晟 彭夏云 韦永强 王英群 胡传活[2] 

机构地区：[1]广西畜禽品种改良站,广西南宁530001 [2]广西大学动物科技学院,广西南宁530005

出　　处：《中国畜牧兽医》2014年第2期170-173,共4页China Animal Husbandry & Veterinary Medicine

基　　金：广西水产畜牧兽医局科技计划项目(桂渔牧科1204934);广西教育厅科研项目(201012MS001)

摘　　要：为了改善猪精液低温保存方法,研究在低温条件下葛根素、酒石酸美托洛尔和二氯乙酸钠3种抗凋亡试剂对保持猪精液品质的作用,采用手握法采集健康公猪精子活力大于0.7的精液,用添加不同浓度葛根素(0、0.02、0.10、0.50、2.50mmol/L)、酒石酸美托洛尔(0、0.02、0.10、0.50、2.50mmol/L)及二氯乙酸钠(0、0.04、0.20、1.00、5.00mmol/L)的稀释液稀释,于5℃下保存,每天检查,以精子稀释后活力、有效存活时间、总存活时间、存活指数及保存72h后精子畸形率和顶体完整率为衡量指标,试验重复6次。结果表明,一定浓度的3种药物能有效地延长低温保存时猪精子的存活时间,其中0.50、0.50及1.00mmol/L分别是葛根素、酒石酸美托洛尔和二氯乙酸钠的最佳添加浓度。The objective of the present study was to improve the low temperature preservation of boar semen and evaluate the effect of three anti-apoptotic drugs （puerarin, metoprolol tartaric and diehloroacetate） on keeping the quality of boar semen. Semen was collected from adult boars of proven fertility with the gloved-hand technique, and only the semen showing a mini- mum of 70% motile was used. Semen was diluted in extenders containing anti-apoptotic drugs （0,0.02,0.10,0.50 and 2.50 mmol/L puerarin, 0,0.02,0.10,0.50 and 2.50 mmol/L metoprolol tartaric acid, 0,0.04,0.20,1.00 and 5.00 mmol/L dichlo- roacetate）. All sperm suspensions were stored at 5 ℃ and sperm motility was detected once a day. Six parameters （motility of extended soermatozoa, spermatozoal-survival effective time, spermatozoal-survival overall time, spermatozoal-survival index, the 72 h deformity rate and acrosomal integrity） were measured, six replicates were performed. The results showed that under the conditions of this study, a certain concentration of three drugs can extend the life-span of spermatozoa under the low tempera- ture, and the optimal concentration of puerarin, metoprolol tartaric acid and dichloroacetate was 0.50, 0 50 and 1.00 retool/L, respectively.

关 键 词：猪 精液 低温保存 抗凋亡试剂 

分 类 号：S814[农业科学—畜牧学]