猪圆环病毒2型流行毒株FF株的分离与鉴定  

Isolation and Identification of Porcine Circovirus Type 2 FF Strain

作　　者：尹秀凤 丁美娟 秦进进 张小飞 

机构地区：[1]南京天邦生物科技有限公司,江苏南京211102

出　　处：《中国畜牧兽医》2014年第2期229-231,共3页China Animal Husbandry & Veterinary Medicine

基　　金：江苏省科技成果转化专项资金(BA2010021)

摘　　要：从临床送检的表现为断奶仔猪多系统衰竭综合征(PMWS)的病死猪肾脏和腹股沟淋巴结病料中,扩增猪圆环病毒2型(porcine circovirus type 2,PCV2)ORF2基因,证明为PCV2阳性病料后,将病料处理后接种到无PCV1污染的PK-15细胞进行分离培养,并连续传代10次,将第10代细胞培养物用PCV2的特异性引物能扩增出预期目的条带,将第10代细胞培养物接种PK-15细胞中,培养72h后用间接免疫荧光检测,结果显示细胞核中有较强的亮绿荧光,表明南京天邦生物科技有限公司生物技术研究所成功分离到1株PCV2,命名为FF株。The materials from the clinical samples of kidney and inguinal lymph nodes characterized by postweaning multi- systemic wasting syndrome （PMWS） of the pigs,proved to be porcine cireovirus type 2 （PCV2） positive samples through the amplification of ORF2 gene of PCV2, were inoculated into the PK-15 cells （PCV1 free） and serially passaged 10 times. Identifi cations were done by PCR method and immunofluorescent assay of the tenth generation of cell cultures, and the specific band could be amplified and a strong bright green fluorescence in the nucleus of PK-15 cells could be detected. The results demonstra- ted that the isolated strain named as FF strain belonged to PCV2.

关 键 词：猪圆环病毒2型 分离 鉴定 FF株 

分 类 号：S852.65[农业科学—基础兽医学]