Lysobacter antibioticus 13-1菌株抗菌物质鉴定及对水稻白叶枯病的防治效果  

Control of rice bacterial leaf blight by antibacterial substances from Lysobacter antibioticus strain 13-1

作　　者：魏兰芳[1] 周丽洪[2] 姬广海[2] 王永吉[2] 汪绍雪[2] 

机构地区：[1]云南农业大学农科基础实验教学中心,云南昆明650201 [2]云南农业大学植物保护学院,云南昆明650201

出　　处：《微生物学通报》2014年第2期274-280,共7页Microbiology China

基　　金：国家自然科学基金项目(No.31360002);农业部农业行业科研专项项目(No.201003029;201303015)

摘　　要：【目的】为了明确对水稻白叶枯病具有优质防效的生防细菌——抗生素溶杆菌13-1菌株抗菌物质及对白叶枯病的防治效果。【方法】研究采用高效液相色谱、质谱、13C核磁共振谱、氢核磁共振谱、电喷雾质谱等分析方法。【结果】13-1产生4种抑菌活性组分:6-甲氧基-10-氧基-1-吩嗪醇、吩嗪、吩嗪-1-羧酸及1-羟基-6-甲氧基吩嗪。4种吩嗪类物质对水稻白叶枯病原细菌均具有抑菌活性。田间小区试验表明,菌株13-1发酵液对水稻白叶枯病的防效在60%以上。【结论】研究明确了菌株13-1产生的抗菌物质为吩嗪类物质,这是国内关于吩嗪类物质控制水稻白叶枯病的首次报道。[Objective] To clarify the antibacterial activity of Lysobacter antibioticus strain 13-1 against rice bacterial blight. [Methods] Four antibiotic compounds were isolated from the fermentation extracts of the strain 13-1 by sephadex LH-20 column chromatography, reverse C-18 Silica gel and high performance liquid chromatography （HPLC）. [Results] These purified compounds were identified as 6-Methoxy-1-phenazinol-10-oxide, Phenazine, 1-Phenazinecarboxylic acid and 1-Hydroxy-6-methoxyphenazine by nuclear magnetic resonance （NMR）, ESI-Mass spectrum analysis. Four phenazine analogs strongly inhibited mμLtiple Xoo strains growth. The protective efficacy of strain 13-1 was above 60% in field plot compared to controls. [Conclusion] The antimicrobial substances of strain 13-1 was phenazine substances that have the inhibition potential against rice bacterial blight.

关 键 词：根际细菌13-1 抗细菌物质 水稻白叶枯病 生物防治 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治] S476[农业科学—植物保护]