姜黄素对CNE-2细胞放射敏感性及机制的影响  

Effect of curcumin on radiosensitization of CNE-2 cells and its mechanism

作　　者：王启瑞[1] 范昊宁[1] 殷志新[2] 蔡红兵[1] 邵萌[1] 刁建新[1] 刘远亮[1] 孙学刚[1] 佟丽[1] 范钦[1] 

机构地区：[1]南方医科大学中医药学院,广东广州510515 [2]南方医科大学基因研究所,广东广州510515

出　　处：《中国中药杂志》2014年第3期507-510,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81173616);广东省科技计划项目(2013A03250003);广州市海珠区科技计划项目(2013-cg-29)

摘　　要：目的:探讨姜黄素(Cur)对鼻咽癌细胞CNE-2放射敏感性及机制的影响。方法:克隆形成实验检测姜黄素对细胞放射敏感性的影响,Graph prism 6.0拟合细胞存活曲线;流式细胞术(FCM)分析细胞周期变化;基因芯片技术检测细胞长链非编码RNA表达差异,Real-time PCR验证部分差异表达基因。结果:10μmol·L-1Cur作用24 h后,其放射增敏比1.03,表明低浓度的Cur可以增加鼻咽癌细胞的放射敏感性;FCM显示Cur联合照射组G2期细胞显著增加,S期细胞显著下降。GUCY2GP,H2BFXP,LINC00623等lncRNA在姜黄素组细胞中显著上调,ZRANB2-AS2,LOC100506835,FLJ36000等lncRNA在姜黄素组细胞中显著下调。结论:Cur对人鼻咽癌细胞CNE-2具有放射增敏作用,其机制可能与改变细胞周期的分布和长链非编码RNA的表达有关。Objective： To investigate the effect of curcumin （Cur） on radiosensitivity of nasopharyngeal carcinoma cell CNE-2 and its mechanism. Method： The effect of curcumin on radiosensitivity was determined by the clone formation assay. The cell survival curve was fitted by Graph prism 6. 0. The changes in cell cycle were analyzed by flow cytometry （FCM）. The differential expression of long non-coding RNA was detected by gene chip technology. Part of differentially expressed genes was verified by Real-time PCR. Result： After 10 umol . L -1 Cur had worked for 24 h, its sensitization enhancement ratio was 1.03, indicating that low concentration of curcumin could increase the radiosensitivity of nasopharyngeal carcinoma cells ; FCM displayed a significant increase of G2 phase cells and significant decrease of S phase cells in the Cur combined radiation group. In the Cur group, the GUCY2GP, H2BFXP, LINC00623 lncRNA were significantly up-regnlated and ZRANB2-AS2 LOC100506835, FLJ36000 ]ncRNA were significantly downregulated. Conclusion： Cur has radiosensitizing effect on human nasopharyngeal carcinoma CNE-2 cells. Its mechanism may be related to the changes in the cell cycle distribution and the expression of long non-coding lncRNA.

关 键 词：姜黄素 放射增敏 细胞周期 基因芯片 长链非编码RNA 

分 类 号：R285[医药卫生—中药学]