基于GC-MS代谢组学法研究黄连、生地黄治疗Ⅱ型糖尿病的配伍机制  

Study on mechanism of combined administration of Coptidis Rhizoma and Rehmanniae Radix in treating type Ⅱ diabetes mellitus

作　　者：王静[1] 袁子民[1] 李云兴[1] 孔宏伟[2] 许国旺[2] 

机构地区：[1]辽宁中医药大学药物分析教研室,辽宁大连116600 [2]中国科学院大连化学物理研究所生物技术部,辽宁大连116023

出　　处：《中国中药杂志》2014年第3期526-530,共5页China Journal of Chinese Materia Medica

基　　金：国家科技部中国国际科学与技术合作项目(2009DFA21450);国家自然科学基金项目(20805045)

摘　　要：该研究采用代谢组学方法对黄连、生地黄配伍前后用于Ⅱ型糖尿病治疗的配伍机制进行初步阐明。建立脂肪乳伴腹腔注射链脲佐菌素致大鼠Ⅱ型糖尿病动物模型,比较黄连、生地黄及其不同比例配伍药对的降血糖、降血脂作用。基于气相色谱-质谱联用技术分析黄连、生地黄配伍前后大鼠尿样代谢轨迹,采用主成分分析法(PCA)研究各组间的代谢物组差异。生化指标结果表明,黄连、生地黄配伍前后均能降低高血糖、高甘油三酯、高胆固醇。尿样代谢组的PCA分析结果表明,黄连组最接近于正常组,不同配伍比例黄连、生地黄组没有明显区分。确定12个差异代谢物与Ⅱ型糖尿病相关,与模型组相比较,黄连给药后对其中7个差异代谢物有显著的回调作用。黄连、生地黄及其不同配伍比例药对在治疗Ⅱ型糖尿病时,黄连起到主要作用,是君药。生地黄是臣药,辅助黄连药材发挥药效作用,本研究为阐明中药配伍机制奠定基础。To make a preliminary study on the mechanism of Coptidis Rhizoma（CR） and Rehmanniae Radix（RR） before and after the combined administration in treating type II diabetes mellitus. The type II diabetes animal model in rats was established by fat emulsion and intraperitoneal injection with streptozotocin, in order to compare the hpyerglycemic and hypolipidemic effects of CR, RR and their combined administration of different ratio. The urinary metabolic profiling in rats of Coptidis Rhizoma and Rehmanniae Radix before and after the combined administration was analyzed by using the gas chromatography-mass spectrometry. The differences among groups in metabolome were analyzed by the principal component analysis （PCA）. The biochemical index results indicated that both CR and RR before and after the combined administration could lower high blood glucose, hypertriglyceride and high cholesterol. According to the analytical results of PCA of the rats＇ urine samples, the CR group was the most close to the normal group, with no significant difference in CR and RR group of different combination ratios. Twelve differentiated metabolites were identified to be related to type II diabetes. Compared with the normal group, the CR-treated group showed significant increase in seven differentiated metabolites. Among CR and RR drugs with different combination ratios, CR played a major role and thus acted as the monarch drug. Whereas RR served as the ministerial drug and assisted CR to show the efficacy. This study laid a foundation for the explanation of the combination mechanism of traditional Chinese medicines.

关 键 词：代谢组学 黄连 生地黄 II型糖尿病 配伍机制 

分 类 号：R285.5[医药卫生—中药学]