高效液相色谱法测定三七地上部分γ-氨基丁酸的含量  

Determination of γ-aminobutyric acid in aerial part of Panax notoginseng by HPLC

作　　者：杨晶晶[1,2] 刘英[1,2] 崔秀明[1,2] 曲媛[1,2] 黄璐琦[3] 

机构地区：[1]昆明理工大学生命科学与技术学院,云南昆明650500 [2]昆明理工大学云南道地药材研究开发中心,云南昆明650500 [3]中国中医科学院中药资源中心,北京100700

出　　处：《中国中药杂志》2014年第4期606-609,共4页China Journal of Chinese Materia Medica

基　　金：昆明理工大学自然科学研究基金项目(KKSY201326022);昆明理工大学分析测试基金项目(20130452)

摘　　要：采用高效液相色谱法测定不同产地的三七地上部分中γ-氨基丁酸的含量。色谱条件采用C_(18)柱色谱柱(4.6mm×250 mm,5μm),以乙腈-4.1 g·L^(-1)醋酸钠缓冲液为流动相,检测波长为254 nm。γ-氨基丁酸在0.01~1.03 g·L^(-1)线性范围内线性关系良好;三七茎叶和花中γ-氨基丁酸的平均加样回收率分别为101.7%(RSD 1.1%,n=3)和97.3%(RSD=0.38%,n=3)。结果表明,不同产地的三七茎叶和花样品GABA含量差异较小,但三七花中GABA平均含量高于茎叶,分别为0.49%,0.53%。该方法操作简便、结果稳定,适合于三七地上部分中γ-氨基丁酸含量测定,为三七地上部分资源综合开发提供依据。The content of y-aminobutyric acid in the aerial part of Panax notoginseng in different productive area was deter- mined by using high performance liquid chromatography. HPLC analysis was made on a C18 （4.6 mm × 250 mm ,5 um） with acetonitrile and water containing 4.1 g . L-1 sodium acetate as mobile phase. The detection wavelength was 254 nm. The HPLC method showed good linearity within the range of 0.01 -1.03 g . L-1. The average recovery of GABA in the stems and leaves of P. notoginseng and the flowers of P. notoginseng was 101.7 % （ RSD 1.1%, n = 3 ） and 97.3 % （ RSD 0.38%, n = 3 ）, respectively. The contents of GABA in the samples of different productive areas were not significant different, and the average contents of GABA in the stems and leaves of P. notoginseng and the flowers of P. notoginseng were 0.49% and 0.53%. This method was simple and reliable, and it was suitable for the determination of y-aminobutyrie acid in the aerial part of P. notoginseng.

关 键 词：三七地上部分 y-氨基丁酸(GABA) 异硫氰酸苯酯(PITC) 高效液相色谱 含量测定 

分 类 号：R284.1[医药卫生—中药学]