基于GC-MS和UPLC-Q-TOF-MS的麻黄汤化学成分识别  

Identification of chemical components of Mahuang decoction by GC-MS and UPLC-Q-TOF-MS

作　　者：李睿[1] 曾岑[1] 王平[1] 孟宪丽[1] 曾勇[1] 

机构地区：[1]成都中医药大学药学院,四川成都611137

出　　处：《中国中药杂志》2014年第4期704-709,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81001496);成都中医药大学科技发展基金项目(ZRMS201239)

摘　　要：复方汤剂成分复杂,需要寻找一个高分离度、高稳定性的分析方法进行定性分析。采用梯度萃取法,将麻黄汤水煎液中的化学组分分为脂溶性和水溶性成分进行定性分析。采用GC-MS技术分离麻黄汤乙酸乙酯提取部分化学成分,质谱使用EI离子源,通过NIST MS Search 2.0标准质谱库定性分析,并结合相关文献资料,共鉴定出40个化学成分;采用UPLCQ-TOF-MS技术分离麻黄汤水提取部分化学成分,质谱使用ESI离子源,通过MZmine-2.9.1,Isotope Pattern软件以及质谱裂解规律定性分析,并对照对照品和结合相关文献资料,共鉴定出39个化学成分。该实验利用了GC-MS和UPLC-Q-TOF-MS对化学成分分析范围的差异,以及MS对未知化合物的准确鉴定优势,为麻黄汤药效物质的研究提供了依据,并为复方组分提供了一种简单快捷的分析鉴定方法。Since the polyjuice potion ingredient is complex, we need to develop an analysis method with well separation and high stability to perform qualitative analysis. After dividing chemical components of Mahuang Decoction into fat-soluble and water-soluble constituents by gradient extraction, GC-MS was used to analyzethe chemical components of the ethyl acetate extraction. The results showed that forty compounds had been identified by NIST MS search 2.0 standard mass spectrometry Library and literatures. Next, UP- LC-Q-TOF-MS was applied to idendify the chemical components of the water extraction. The results showed that thirty-nine compounds had been identified by MZmine-2. 9. 1, Isotope Pattern, fragmentation regularity of mass spectrometry and literatures. This experiment will provide evidences for elucidation of the effective substance in Mahuang decoction and can be used as a simple, shortcut method for analysis and identification for the polyjuice potion.

关 键 词：麻黄汤 乙酸乙酯部分 水提取部分 GC—MS UPLC—Q-TOF—MS 

分 类 号：R284[医药卫生—中药学]