两面针根和茎超临界CO2萃取物GC-MS比较分析及体外细胞毒活性评价  

GC-MS analysis and cytotoxic activity of the supercritical extracts from roots and stems of Zanthoxylum nitidum

作　　者：何紫凝[1] 刘嘉炜[1] 李武国[1] 司马贞华 陈蔚文[1] 

机构地区：[1]广州中医药大学中药资源科学与工程研究中心 岭南中药资源教育部重点实验室,广东广州510006

出　　处：《中国中药杂志》2014年第4期710-714,共5页China Journal of Chinese Materia Medica

基　　金：广东省教育部产学研结合项目(2009B090300202);广东省“211工程”三期重点学科建设项目(粤发改社[2009]319号)

摘　　要：利用GC-MS技术从两面针根和茎超临界CO_1萃取物中分别鉴定出31个和51个低极性成分,其中共有成分27个,主要为斯巴醇(根18.49%,茎26.18%)、棕榈酸(根14.24%,茎12.79%)、芳姜黄酮(根6.95%,茎8.88%)、油酸(根8.39%,茎5.71%)和己酸(根4.39%,茎7.78%)等;体外MTT法测试表明两面针根和茎萃取物对人肝癌细胞Huh-7和大鼠小肠隐窝上皮细胞IEC-6均没有明显细胞毒性。实验研究表明两面针根和茎低极性化学成分种类和含量相似,其体外细胞毒活性也相似,可为两面针充分利用野生资源和扩大药用部位提供进一步参考。The volatile components of roots and stems of Zanthoxylum nitidum were investigated by supercritical fluid carbon dioxide extraction （SFE-CO2） and gas chromatography-mass spectrometry（GC-MS）. Thirty-one and fifty-one compounds were identified in the supercritical extracts from roots and stems of Z. nitidum, respectively, and total twenty-seven compounds were the common constituents. Among them, the major constituents in root and stem supercritical extracts were spathulenol （ 18.49 and 26. 18% ）, n-hexadecanoic acid （14. 24% and 12. 79% ）, ar-tumerone （6. 95% and 8.88% ）, oleic acid （8.39% and 5.71% ） and hexanoic acid （4. 39% and 7. 78% ）. The in-vitro MTT assay showed that the volatile components of roots and stems of Z. nitidum did not exhibited any cytotoxic activity against human cancer Huh-7 and normal IEC-6 cells. These results indicated the same nature of the volatile constituents in the root and stem of Z. nitidum. This investigation may provide further evidence for expansion of medicinal parts of Z. nitidum.

关 键 词：两面针 低极性成分 超临界CO2萃取 GC—MS 细胞毒性 

分 类 号：R284[医药卫生—中药学]