高效液相色谱／氢化物发生-原子荧光光谱法检测微藻中的砷形态  

Detection of Arsenic Species in Microalga Using High Performance Liquid Chromatography/Hydride Generation-Atomic Fluorescence Spectrometry

作　　者：王亚[1] 张春华[2] 申连玉 王淑[1] 葛滢[1] 

机构地区：[1]南京农业大学资源与环境科学学院、江苏省海洋生物学重点实验室,江苏南京210095 [2]南京农业大学生命科学实验中心,江苏南京210095

出　　处：《分析科学学报》2014年第1期21-25,共5页Journal of Analytical Science

基　　金：国家自然科学基金(No.41371468);国家重大科技支撑项目(No.2011BAD13B09);江苏省海洋生物学重点实验室开放课题(No.JSMK2011-002)

摘　　要：建立了盐藻和螺旋藻中砷形态的高效液相色谱／氢化物发生一原子荧光光谱（HPLC／HG—AFS）分析方法。优化了微藻中砷形态的提取方法、流动相浓度及PH、KBH1浓度、载流浓度、AFS参数。优化后砷形态的提取效率范围为97．3％～99．1％，在优化后的实验条件下，As（Ⅲ）、二甲基砷酸（DMA）、一甲基砷酸（MMA）和As（V）的线性相关系数分别为0．9999、0．9996、0．9997和o．9994，检出限分别为0．19、0．40、0．34、0．52μg／L，盐藻的砷加标回收率为91．5％～100．8％，螺旋藻的砷加标回收率为95．6％～104．59／6。研究结果表明，该方法检测快速（10min），且测得微藻中的砷形态数据准确、可靠。A method using high performance liquid chromatography-hydride generation-atomic fluorescence spectrometry（HPLC/HG-AFS） to determine arsenic speciation in Dunaliella tertiolecta and Spirulina platensis has been developed. Various operating parameters including extraction procedures of arsenic species in microalgae, the concentration of mobile phase and pH, potassium borohydride solution, carrier solution and the AFS conditions were optimized. Under the optimal experimental conditions, extraction efficiency in the range of 97. 3%-99. 1%, and the linear correlation coefficients of 0. 9999, 0. 9996,0. 9997 and 0. 9994 for As（ Ⅲ ）, dimethyl-arsonic acid（DMA）, monomethyl-arsonic acid（MMA） and As（V ）,and the limits of detection of 0.19,0. 40,0. 34 and 0.52 μg/L were obtained, respectively. The recoveries of arsenic species for spiked Dunaliella tertiolecta were about 91.5%-100.8%, and the recoveries of arsenic species for spiked Spirulina platensis were about 95. 6%. 104.5%. The results indicated that HPLC-HG-AFS was a reliable method for fast determination（10 min） of arsenic species in microalgae.

关 键 词：盐藻 螺旋藻 砷形态 高效液相色谱 氢化物发生-原子荧光光谱法 

分 类 号：O657.31[理学—分析化学]