头孢曲松钠与1，2-萘醌-4-磺酸钠的光度分析及应用  

Spectrophotometric Analysis of Ceftriaxone Sodium with Sodium 1,2-Naphthoquinone-4-Sulfonate as Chromogenic Agent

作　　者：范秉琳[1,2] 倪天军[2] 袁建梅[2] 程迪[2] 李全民[1] 

机构地区：[1]河南师范大学化学与环境科学学院、河南省环境污染控制重点实验室,河南新乡453007 [2]新乡医学院基础医学院,河南新乡453003

出　　处：《分析科学学报》2014年第1期91-94,共4页Journal of Analytical Science

基　　金：河南省基础与前沿技术研究计划(No.122300410412,132300410205)

摘　　要：建立了用1，2-秦醌-4-磺酸钠（NQS）作为显色剂，分光光度法测定头孢曲松钠的新方法。在pH=13．0的缓冲溶液中，NQS与头孢曲松钠发生亲核取代反应生成1：1的红褐色配合物，其最大吸收波长488nm。方法线性范围为2．4～168mg／L，相关系数r=0．9993，检出限为1．3mg／L。该法成功应用于针剂中头孢曲松钠含量的测定，回收率在98．29／6～101．6％。A new method was established to determine ceftriaxone sodium by using sodium 1, 2- naphthoquinone-4-sulfonate（NQS） as a ehromogenic reagent. A russet product can be formed owing to the nucleophilic substitution reaction between ceftriaxone sodium and NQS at pH = 13.0. The maximal absorption wavelength of the product is 488 nm. Absorbency was linear with the concentration of ceftriaxone sodium in the range of 2. 4-168 mg/L with a correlation coefficient of r = 0. 9993. The detection limit was 1.3 mg/L and the average recovery rate was in the range of 98.2%-101.6%. The results indicated that the method could be applied to the determination of ceftriaxone sodium in injection.

关 键 词：1 2-萘醌-4-磺酸钠 头孢曲松钠 分光光度法 

分 类 号：O657.32[理学—分析化学]