基质固相分散-高效液相色谱法测定大青叶中靛玉红  

Determination of Indirubin in Leaves of Isatis indigotica Fort.Using Matrix Solid-Phase Dispersion Coupled with High Performance Liquid Chromatography

作　　者：刘贺[1] 于爱民[2] 张寒琦[2] 

机构地区：[1]吉林省环境监测中心站,吉林长春130011 [2]吉林大学化学学院,吉林长春130012

出　　处：《分析科学学报》2014年第1期115-118,共4页Journal of Analytical Science

摘　　要：将基质固相分散提取技术应用于大青叶中靛玉红的提取。对分散剂和洗脱溶剂等实验因素进行了考察和优化。结果表明，以硅胶为分散剂，甲醇为洗脱溶剂时，靛玉红的提取效果最好。实验采用高效液相色谱对提取液进行分析。线性范围为0．0151～12．5μg·mL-1，线性相关系数0．9999。方法的精密度小于3．2％。平均回收率为93．97％～100．9％。将优化的方法与索氏和超声提取法进行了对比，结果表明，由MSPD获得的靛玉红产率与索氏法获得的相当。建立的方法快速，适合分析大青叶中的靛玉红。A method based on matrix solid-phase dispersion （MSPD） has been developed for the extraction of indirubin in the leaves of Isatis indigotica Fort. The sample was blended with silicon gel and the resulting mixture was placed in extraction column and then eluted with methanol. Analysis was performed by high performance liquid chromatography （HPLC）. Several dispersion adsorbents and elution solvents were tested in order to obtain the highest yields. The responce of the active component is linear in concentration range of 0. 0151 to 12.5 μg·mL-1 , with the correlation coefficient of 0. 9999. The precision（relative standard deviation） is lower than 3.2%,and mean recoveries range from 93.97% to 100.9 %. The present method was compared with ultrasonic and Soxhlet extraction and the results showed that the indirubin yield obtained by MSPD is comparable to these obtained by ultrasonic and Soxhlet extraction.

关 键 词：基质固相分散提取 高效液相色谱 靛玉红 

分 类 号：O657.72[理学—分析化学]