液质联用法分析王老吉凉茶中的3种有效成分  

Simultaneous determination of three effective components in Wanglaoji cool tea by ultra performance liquid chromatography and tandem mass spectroscopy

作　　者：朱平川[1] 岑卫健[1] 范晓苏[2] 

机构地区：[1]亚热带农业生物资源保护与利用国家重点实验室(广西大学),广西南宁530004 [2]广西大学化学化工学院,广西南宁530004

出　　处：《湖北大学学报（自然科学版）》2014年第2期123-126,131,共5页Journal of Hubei University：Natural Science

基　　金：广西自然科学基金项目(桂科自0832034);广西大学科研基金资助项目(XJZ120280)资助

摘　　要：建立同时测定王老吉凉茶中的绿原酸、异绿原酸C、甘草酸3种有效成分含量的超高效液相色谱一串联质谱（UPLC-MS／MS）分析方法．用Agilent Zorbax RRHD Eclipse PlusC18（50mm×2．1mm，1．8μm）色谱柱，以甲醇-0．1％甲酸的水溶液为流动相，流速为0．30mL／min在电喷雾电离（ESI）模式下，采用的是多重反应监测模式（MRM）进行检测，绿原酸、异绿原酸C、甘草酸的线性范围分别为0．030～4．500mg／L、0．045～4．500mg／L、0．016～2．000mg／L；检出限分别为10、12、3μg／L；3种成分的加样回收率为94．7％～103％，RSD均不大于1．9％.该方法简便、准确、快速、高灵敏度，已成功地用于实际的样品分析．A method for simultaneous determination of chlorogenic acid, isochlorogenic acid C, glycyrrhizic acid in wanglaoji cool tea was established by ultra performance liquid chromatography with tandem mass spectrometry （UPLC-MS/MS）. The UPLC separation was performed on a Zorbax RRHD Eclipse Plus C18 column （2. 1 mm×50 mm, 1.8μm） by using methanol and 0.1% formic acid aqueous solution as mobile phase with the gradient elution at a flow rate of 0.30 mL/min. The analytes were detected by tandem mass spectrometry with the ESI source and carried out under the multiple reaction monitoring （MRM） mode. Under the optimum conditions, the calibration curves were linear in the range of 0.030-4.500 mg/L for chlorogenic acid, 0.045- 4.500 mg/L for isochlorogenic acid C, 0. 016-2. 000 mg/L for glycyrrhizic acid, and the detection limits was 10,12, 3 μg/L, respectively. The average recoveries of the three effective components were between 94.7% and 103%, with all relative standard deviations not more than 1.9%. The developed method was simple, rapid and accurate, and suitable for the quality control of the three components in wanglaoji cool tea.

关 键 词：王老吉凉茶 有效成分 超高效液相色谱一串联质谱(UPLC-MS MS) 

分 类 号：O657[理学—分析化学]