血清炎症因子在OLETF鼠2型糖尿病发病过程中的动态变化  

Dynamic change of serum inflammatory markers during the development of diabetes in OLETF rats

作　　者：朱波[1] 薛耀明[1] 张燕[1] 李晨钟[1] 潘永华[1] 王玲[1] 何飞英[1] 

机构地区：[1]南方医科大学南方医院内分泌代谢科,广州510515

出　　处：《中国糖尿病杂志》2014年第2期157-160,共4页Chinese Journal of Diabetes

基　　金：国家自然科学基金(30371839);广东省中医药局科研课题(2009454);广东省重点科技攻关项目(社会发展公关2003C34424);广东省科技计划项目(2004B30701011)

摘　　要：目的探讨自发性T2DM大鼠模型OLETF鼠发病过程中炎症因子与IR的关系。方法 20只雄性OLETF鼠(OLETF组)和10只雄性LETO鼠(LETO组)在8、32和40周龄被分批处死。检测血糖和血浆胰岛素水平。免疫散射比浊法检测各组血清高敏C反应蛋白(hsC-RP),ELISA检测血清TNF-α、IL-6、单核细胞趋化蛋白1(MCP-1)水平,并计算ISI。结果 8周龄时,OLETF组血清hsC-RP和MCP-1均高于LETO组;随周龄增加,OLETF组血清hsC-RP、TNF-α、IL-6、MCP-1水平逐渐升高,且40周龄时,均高于LETO组,仅ISI低于LETO组(P<0.01)。32周龄和40周龄时,ISI与hsC-RP、IL-6呈负相关(P<0.01),血清各炎症因子之间互为正相关(P<0.05)。结论 OLETF鼠血清炎症因子在糖尿病发生前即升高,并随病情进展持续升高,与IR密切相关,参与T2DM的发生发展。Objective To analyze the dynamic expression of serum inflammatory markers during the development of diabetes in OLETF rats. Methods 20 male OLETF rats （OLETF group） and 10 male LETO （LETO group） rats were executed separately at age of 8, 32 and 40 weeks. The fasting blood glucose and plasm insulin were determined. Serum hsC-RP was determined by the immunonephelometry assay. Serum TNF-α, IL-6, MCP-1 were measured by enzyme-linked immunoadsordent assay （ELISA） and insulin sensitivity index （ISI） were calculated. Results The serum hsC-RP and MCP 1 in OLETF rats were significantly higher than LETO rats at 8-week age. The levels of serum hsC-RP, TNF-α, IL-6, MCP-1 in OLETF group were increased with week age increasing, and were higher than LETO group at 40-weeks age, but ISI was lower（P〈0.01）. ISI was negatively correlated with hsC-RP and IL-6 at rats of 32 and 40 weeks of age（P〈0. 01 ）. Between the inflammatory markers there was positive correlatlon（P〈0. 05）. Conclusion Serum inflammatory markers increased in OLETF rats before the onset of type 2 diabetes metlitus, and along with T2DM progression, closely correlated with insulin resistance and contribute to the onset and development of type 2 diabetes mellitus.

关 键 词：炎症因子 胰岛素抵抗 糖尿病 OLETF鼠 

分 类 号：R587.1[医药卫生—内分泌]