腹腔注射分泌胰升血糖素样肽-1重组腺伴随病毒对db/db小鼠高血糖的影响  

Regulation effect of secretory glucagon-like peptide 1 recombinanted to adeno-associated virus on hyperglycemia in db/db mice:A experimental study

作　　者：娄明武[1] 梁冰[1] 张明东[1] 

机构地区：[1]深圳市龙岗中心医院医学影像科,518116

出　　处：《中国糖尿病杂志》2014年第2期174-176,共3页Chinese Journal of Diabetes

基　　金：广东省科学技术厅资助项目(2005B36001082);深圳市科技和信息局资助项目(JH200505260306A);深圳市龙岗区专家提升计划资助项目(卫生类7号)

摘　　要：目的 观察腹腔注射分泌表达胰升血糖素样肽-1（GLP-1）的重组腺伴随病毒对db/db小鼠高血糖的纠正作用. 方法 将12只8周龄db/db小鼠随机分为对照组与实验组.测量体重、血糖.实验组腹腔注射分泌表达GLP-1的重组腺伴随病毒,对照组腹腔注射对照病毒.给药后分别于第2、4、6、8、10、12周测量体重,并检测血糖水平. 结果 随观察时间的延长,对照组体重、血糖呈上升趋势,实验组则下降.实验组2周时,体重（38.3±4.4）g,血糖（18.2±3.0）mmol/L,与对照组[（44.2±4.7）g、（22.6±4.5）mmol/L]比较,差异无统计学意义（P＞0.05）.第4～12周时实验组体重和血糖均低于对照组,两组间比较差异有统计学意义（P＜0.05）. 结论 通过腹腔注射分泌表达GLP-1的重组腺伴随病毒方式,可纠正db/db小鼠高血糖.Objective To study the regulation effect of intraperitoneal injection of the secretory glucagon-like peptide 1 （GLP-1） recombinant adeno-associated virus on hyperglycemia in db/db mice with defective leptin receptor. Methods Twelve eight-week old db/db mice were divided into control and experimental groups randomly（n= 15, each）. After measuring weight and blood glucose in two groups, the secretory GLP-1 recombinant adeno-associated virus（ 1 × 10^7 pfu, qd） were intraperitoneally injected into experimental group for twice. And the control group were intraperitoneally injected with control virus （ 1 ×107 pfu, qd）. Weight and blood glucose were measured in two groups at 2, 4, 6, 8, 10, 12 weeks after the final intraperitoneal injection. Results Two weeks after injection, weight and blood glucose were all increased in control group, but decreased in experimental group along with time going, there was no obviously differences in weight and blood glucose between two groups（P〉0. 05）. But the difference in the indexes between two groups was obvious at time points of 4, 6, 8, 10, 12 weeks. Conclusion The intraperitoneal injection of the secretory GLP-1 recombinant adeno-associated virus has regulation effect on high blood glucose in db/db mice.

关 键 词：胰升血糖素样肽-1 依赖病毒 DB db小鼠 

分 类 号：R587.1[医药卫生—内分泌]