成分分离机制备冷沉淀凝血因子  

Investigation of cryoprecipitate preparation using automated blood component separator

作　　者：王飞[1] 王颖[1] 李一鸣[1] 陈丽婷[1] 陈葆欣 

机构地区：[1]深圳市血液中心,广东深圳518035

出　　处：《中国输血杂志》2014年第1期42-44,共3页Chinese Journal of Blood Transfusion

摘　　要：目的采用成分分离机制备冷沉淀,探讨冷沉淀质量是否符合国家标准[1],同时实现冷沉淀制备过程的可追溯性。方法将96袋新鲜冰冻血浆分为2组,每组各48袋,一组血浆在4℃恒温水浴摇摆条件下融化,采用虹吸原理手工制备冷沉淀凝血因子;另一组血浆在4℃恒温水浴摇摆条件下融化约60 min后离心,使用成分分离机自动分离冷沉淀凝血因子。结果虹吸法和离心法制备冷沉淀凝血Ⅷ因子含量分别为(101.1±25.4)IU和(102.5±20.6)IU,t=0.178,P>0.05;合格率分别为60.42%和100%,χ2=20.463a,P<0.05;纤维蛋白原含量分别为(220.6±69.3)mg和(264.8±70.5)mg,合格率分别为83.33%和100%,χ2=8.727a,P<0.05。结论成分分离机制备的冷沉淀质量优于虹吸法,产品质量合格率高,同时能够实现制备过程的信息化管理。Objective Cryoprecipitate were made using ftrlly automated blood component separator [ SEPAMATIC-SL （ III ） 1. The quality of eryoprecipitate was investigated to meet the national quality standard. Meanwhile, the processing of cryoprecipitate preparation was able to be retrospected. Methods 96 units of fresh frozen plasma were randomly divided in- to two groups. Cryopreeipitate was prepared in a traditional manual method for one group （called manual group） and cryo- precipitate was made using fully automated blood component separator （called machine group） for another group. Cryoprecipitate factor and fibfinogen were tested for each product. Results The levels of eryopreeipitate VIII factor were（ 101.1 ± 25.4 ）IU and（ 102.5 ± 20.6 ）IU individually for manual group and machine group. The differences were of no statistical significance （n = 48, t = 0. 178 ,P 〉 0. 05 ）. The qualified product rates were 60.42% and 100% for manual and machine group respectively. The differences were of statistlcal significance （ n = 48 ,X2 = 20.463 a, P 〈 0.05 ）. The levels of fibrinogen were （220.6 ± 69.3 ） mg and （ 264.8 ± 70.5 ） mg for manual group and machine group respectively. The qualified product rates were 83.33% and 100% respectively. The differenees were of statistical significance （ n = 48 ,X2 = 8. 727a, P 〈0. 05 ）. Conclusion Cryopreeipitate was of better quality prepared by machine than made manually. Cryopreeipitate was of higher quality product rate as well. Furthermore, the whole preparation process could be computerized.

关 键 词：冷沉淀 质量 虹吸法 机器法 自动分离 合格率 

分 类 号：R457.1[医药卫生—治疗学]