阿的平对高功率微波损伤小鼠睾丸的保护作用  

Effect of quinacrine on injury by microwave irradiation in mice testis

作　　者：吴燕[1] 刘淑红[1] 旦增[2] 索朗曲宗[2] 林凯[3] 周红梅[4] 苏振涛[4] 葛学铭[1] 格桑罗布[2] 赵永岐[1] 范明[1] 

机构地区：[1]军事医学科学院基础医学研究所,北京100850 [2]西藏自治区人民医院 [3]空军总医院临床检验中心 [4]军事医学科学院放射医学研究所

出　　处：《中国计划生育学杂志》2014年第2期84-87,共4页Chinese Journal of Family Planning

基　　金：国家自然科学基金项目(31071042);军队医药科研"十五"专项课题(01L024);中国博士后科学基金(20100480774)

摘　　要：目的:探讨阿的平对高功率微波辐射小鼠睾丸的保护作用。方法:雄性小鼠随机分为4组,即正常组、辐射对照组、阿的平低剂量组(12.6mg/kg)、阿的平高剂量组(50.4mg/kg)。小鼠分别灌胃给予注射用水和不同剂量阿的平后,使用频率2.856GHz、脉宽500ns的微波源,进行50mW/cm2的微波远场照射30min。于照射后即刻、1d、2d、7d取小鼠睾丸。将小鼠睾丸组织病理切片观察其形态学变化;组织蛋白匀浆法提取睾丸组织蛋白检测热休克蛋白(HSP)70表达变化。结果:微波照射小鼠30min后,辐射对照组的睾丸组织从即刻至7d均有病理改变,曲精小管边缘不整齐,生精细胞水肿,排列紊乱,间质充血。给药组与对照组相比曲精小管排列基本整齐,边缘清晰,生精上皮细胞肿胀较对照组减轻。其中给药低剂量组在照射即刻至2d改善效果与高剂量组相似,但在7d时高剂量组的改善效果明显优于低剂量组。Western blot结果显示,在照后2d阿的平高、低剂量给药组与辐射对照组相比HSP70蛋白表达升高,在7d时只有阿的平高剂量组与辐射对照组相比HSP70蛋白表达升高。结论:阿的平可能是通过上调HSP70蛋白的表达,发挥其抗炎和抗凋亡作用,从而对高功率微波致伤的小鼠睾丸组织产生保护作用。Objective： To explore the effect of quinacrine on testis injury induced by microwave irradiation in mice. Methods： Male mice were randomly divided into four groups, namely normal control group, radiation control group, low--dose of quinacrine group （12.6mg/kg） and high--dose of quinacrine group （50.4mg/kg）. Quinacrine was orally given to mice one hour before microwave irradiation, and except normal control group, all animals received 50mW/cm2 microwave irradiation for 30 minutes. Immediately after irradiation, and 1 day, 2 days, 7 days after irradiation, testis injury of mice was detected by the pathophysiologic method, while, pathophysiologie detection for animals of normal control group was finished 7 days after irradiation experiment. Meanwhile, the expression leval of heat shock protein （HSP） 70 in mice testis was analyzed using Western Blot. Results： The pathological examination showed that the microwave irradiation induced seminiferous tubules irregular shape, spermatogenic cells edema and irregular arrangement, as well as interstitial hyperemia. While in quinacrine groups, spermatogenic cells arranged in rows, and suffered less swelling compared with the radiation control group. Moreover, the microwave irradiation resulted in the increased level of HSPT0 expression in mice testis, while, quinacrine pretreatment further up--regulated the expres- sion level of HSP70. Conclusion： The protection from testis injury with microwave irradiation by quinacrine may be re- lated to anti--inflammatory and anti--apoptosis effects through the increased level of HSPT0 expression.

关 键 词：阿的平 微波辐射 睾丸损伤 保护作用 

分 类 号：R965[医药卫生—药理学]