一个犬凝集素基因VIP36的人类同源基因cDNA的克隆、染色体定位和表达谱分析  

Cloning, Mapping and Expression Analysis of a Human Homologous cDNA of Dog Lectin Gene, VIP 36

作　　者：汪玮[1] 曾立[1] 刘建平[1] 唐榕[1] 顾少华[1] 方国洪 谢毅[1] 毛裕民[1] 

机构地区：[1]复旦大学生命科学学院遗传学研究所,上海200433

出　　处：《复旦学报（自然科学版）》2000年第6期588-592,共5页Journal of Fudan University：Natural Science

基　　金：国家自然科学基金资助项目!(39870 40 2 )

摘　　要：从人的胎脑cDNA文库中克隆到一条犬凝集素基因VIP 36的人类同源基因 .此cDNA序列全长 2 430bp ,拟编码一个 382个氨基酸残基的蛋白 .它编码的蛋白与犬VIP 36有 5 2 %的同源性 ,因此将其命名为人类VIP36L基因 .应用辐射杂交方法 ,将该基因定位在人 2号染色体的分子标记D2S388和D2S113之间 .采用基因芯片杂交的方法研究其表达谱情况 。A human homolog of dog lectin gene, VIP 36, was cloned from human fetal brain cDNA library. The 2 430 bp cDNA encodes a protein with 382 amino acids. The putative protein shows 52 % identity to VIP36 protein, so this gene is termed human VIP 36 L in agreement with the HUGO Nomenclature Committee. By using radiation hybrid mapping, the VIP 36 L gene is localized to human chromosome 2 between molecular markers D2S388 and D2S113. The relative tissue distribution of the VIP 36 L mRNA was determined by DNA microarray. From the results, the VIP 36 L mRNA was found in human fetal skin and liver cancer at higher level.

关 键 词：VIP36L基因 囊泡结合蛋白 辐射杂交 基因芯片 凝集素基因表达谱 克隆 

分 类 号：Q75[生物学—分子生物学] Q78