新的人羧肽酶A抑制物cDNA的克隆、定位和表达谱分析  

Cloning, Mapping and Expression Analysis of a Novel Human Carboxypeptidase A Inhibitor Gene

作　　者：赵炜[1] 李欣 蔺颖 袁元 宋晨曦 薛波 谢毅[1] 毛裕民[1] 

机构地区：[1]复旦大学生命科学学院遗传学研究所,上海200433 [2]上海博容基因开发有限公司,上海200433

出　　处：《复旦学报（自然科学版）》2000年第6期609-612,共4页Journal of Fudan University：Natural Science

基　　金：上海市现代生物与新药产业发展基金资助项目!(98431912 1);国家科技部科技型中小企业创新基金资助项目!(0 0C2 6 2 1310 0 16 5 )

摘　　要：从人的胎脑cDNA文库中 ,克隆到一条全新的人类羧肽酶A抑制物基因 .此cDNA序列全长 10 49bp ,包括翻译起始位点附近的Kozak序列、6 6 9bp的开放读框以及 3′端的加尾信号 ,共编码 2 2 2个氨基酸 .它编码的蛋白与小鼠Latexin蛋白和大鼠Latexin蛋白分别有高达 85 %和 84%的同源性 ,因此命名为人类LATEXIN基因 .应用辐射杂交方法 ,将该基因定位在人 3号染色体 3q2 4区段 ,位于分子标记D3S16 0 5和D3S15 5 3之间 .采用基因芯片杂交的方法研究其在某些组织或细胞中的表达情况 ,发现在前列腺癌中表达量较高 .A novel human carboxypeptidase A inhibitor gene was cloned from human fetal brain cDNA library. The 1 049 bp cDNA sequence spans an open reading frame of 669 bp, encoding a protein with 222 amino acids. The putative protein shows 85% and 84% identity to Latexin protein in mouse and rat respectively, so this gene was termed human LATEXIN gene in agreement with the HUGO Nomenclature Committee. By radiation hybrid mapping, the LATEXIN Gene was localized to human chromosome 3q24 between molecular markers D3S1605 and D3S1553. The relative tissue distribution of LATEXIN gene was determined by DNA microarray, from which a high signal was found in human prostate cancer.

关 键 词：LAtexin蛋白 羧肽酶A抑制剂 辐射杂交 基因芯片 克隆 表达 

分 类 号：Q51[生物学—生物化学] Q753