PMA激活内皮细胞早期应答基因谱分析  

Global Expression Analysis of Endothelial Early Response Gene induced by PMA

作　　者：王曙[1] 和桂芬 陈沁 吴超群[1] 李瑶[1] 刘建平[1] 谢毅[1] 毛裕民[1] 

机构地区：[1]复旦大学生命科学学院遗传学研究所,上海200433 [2]博道基因有限公司,上海200433

出　　处：《复旦学报（自然科学版）》2000年第6期651-655,共5页Journal of Fudan University：Natural Science

基　　金：上海现代生物与新药产业发展基金资助项目!(98431912 1);国家科技部科技型中小企业创新基金资助项目!(0 0C2 6 2 1310 0 16 5 )

摘　　要：血管内皮细胞具有一系列重要生理功能 ,内皮细胞受外界刺激时可转录众多的mRNA ,体现了它们的生物学重要性 .内皮细胞的极早期应答基因 (Immediateearlyresponsegene ,ERG)是一类重要的介导基因 ,决定了外界刺激条件下细胞的最后反应模式 .以包含 40 96条各种人体基因的DNA芯片观察了血管内皮细胞受分化诱导剂PMA(phorbolmyristateacetate)激活后早期应答基因的表达谱 ,证实 17条ERG属已知基因 .数据处理聚类结果表明 ,多数ERG属蛋白质激酶及转录调控因子 ,2 3条上调新基因从功能上归类为ERG ;并且报道了其中的一条新基因的序列特征及其染色体位置和基因结构 ,为研究这个基因的功能提供了有力的实验依据 .Endothelial cells play important physiological roles, the identity and abundance of mRNA species within endothelial cells indicate their biological potential. The induction of immediate early responses genes (ERG) in endothelials is critical to determining the ultimate outcome of exposure to mitogen. The response of endothelial cells to PMA, was explored with a complementary DNA microarray representing about 4 096 different human genes. Interestingly, most of the known genes (13/17) upregulated by PMA are involved in signal transduction or kinases. 23 new genes are also upregulated and belong to ERG functionally. The sequence character, chromosome location and structure of one of the upregulated new genes are rsported, providing compelling experimnetal evidence to further research of this gene's function.

关 键 词：DNA芯片 血管内皮细胞 信号传导 转录因子 染色体定位 极早期应答基因谱 

分 类 号：Q754[生物学—分子生物学] R331.32[医药卫生—人体生理学]