一条在人睾丸中高表达的新的环指蛋白(RNF20)  

RNF20,a Human Novel RING Finger Protein Expressed High in the Testis Located in 9q22.1-22.3

作　　者：吴海[1] 应康[1] 顾少华[1] 夏放[1] 谢毅[1] 杨泉胜[2] 梅举[3] 黄盛东[3] 

机构地区：[1]复旦大学生命科学学院遗传学研究所遗传工程国家重点实验室,上海200433 [2]浙江大学生物系,浙江杭州310027 [3]第二军医大学第一附属医院,上海200433

出　　处：《复旦学报（自然科学版）》2000年第6期663-666,共4页Journal of Fudan University：Natural Science

基　　金：上海市现代生物与新药产业发展基金资助项目!(98431912 1)

摘　　要：从人胎脑构建的cDNA文库中 ,通过大规模测序筛选的方法获得一条新基因 .该基因蛋白序列含有 1个环指 (RINGfinger)基序及 2个进核信号 (nuclearlocalizationsignal,NLS)序列 ,将此基因命名为RNF2 0 (RINGFinger 2 0 ) .用放射杂交细胞系 (RadialhybridRH)方法定位此基因在人染色体的 9q2 2上 .Northern杂交表明 ,其mRNA在睾丸组织中高表达 .用小鼠睾丸做原位杂交表明 ,小鼠的同源基因在精原细胞及初级精母细胞中高表达 ,未成年小鼠睾丸中的精原细胞没有表达 ,提示RNF2 0可能参与精子发生的调控作用 .A novel RING finger gene was isolated from screening a human fetal brain cDNA library. In additional, two bipartite nuclear localization signals(NLS BP) were found in its protein sequence. The gene was located at chromosome 9q22 through RH (radiation hybird) mapping. The result of Northern blot using MTN (multi tissue Northern) indicated that it expressed in testis at very high level however, at very low lever in prostate and ovary. In situ hybridization of mouse testis indicated that the mouse homology of the RING finger gene was expressed high in spermatogonia and primary spermatocyte. These suggested that the RING finger gene was important in spermatogenesis.

关 键 词：环指蛋白 原位杂交 精子发生 人睾丸 

分 类 号：R339.21[医药卫生—人体生理学] Q954.434[医药卫生—基础医学]