锌指结构基因ZNF302序列的克隆与表达谱分析  

Cloning and Tissue-Expression Pattern Analysis of a C_2H_2 Zinc Finger ZNF302 cDNA

作　　者：夏放[1] 杨泉胜[2] 应康[1] 戴建凉[1] 顾少华[1] 谢毅[1] 毛裕民[1] 

机构地区：[1]复旦大学生命科学学院遗传学研究所,上海200433 [2]浙江大学生物系,浙江杭州310027

出　　处：《复旦学报（自然科学版）》2000年第6期667-670,共4页Journal of Fudan University：Natural Science

基　　金：上海市现代生物与新药产业发展基金资助项目!(98431912 1)

摘　　要：从人胎脑cDNA文库中克隆到 1条 16 85nt的锌指蛋白新基因cDNA序列 ,命名为ZNF30 2 ;生物信息学分析表明 ,ZNF30 2的C末端含有 13个保守的C2 H2 锌指结构 ;Northern blot结果提示这一基因可能存在 3 .0kb和 4.2kb的 2种转录本 ;Unigene软件包分析 ,该基因定位于 19号染色体 19q13 .2 13 .3 ;cDNA基因芯片检测显示疤痕成纤维细胞经TGFβ1刺激和ECV30 4细胞经PMA ,LPS刺激后 ,ZNF30 2基因表达明显升高 ,提示其与细胞增殖的正调控有关 .A 1 685 nt cDNA of zinc finger (ZNF) gene was cloned from a human fetal brain cDNA library and named as ZNF 302. Bioinformatic analysis indicates that ZNF 302 contains 13 highly conserved C 2H 2 type zinc finger motifs at the C terminus. Northern blot analysis indicates there are two transcripts of ZNF 302 with 3.0 kb and 4.2 kb respectively. The gene was positioned to chromosome 19q13.2 13.3 by Unigene assay. The expression level of ZNF 302 is higher in keloid fibroblasts stimulated by TGF β 1 and in ECV304 cells stimulated by PMA and LPS than that in normal control by using cDNA microarray. It suggests ZNF 302 might be associated with the up regulation of cell proliferation.

关 键 词：锌指蛋白 生物信息学 基因芯片 锌指结构基因 ZNF302 序列分析 表达谱 

分 类 号：Q754[生物学—分子生物学] Q591.2