人脐带间充质干细胞对输卵管炎大鼠输卵管超微结构和功能的改变  

Oviduct Ultrastructure and Function Comparison of Effectiveness of WJMSCs on Rat's Acute and Chronic Salpingitis

作　　者：罗惠娟[1] 周娟[1] 汤小湄[1] 肖小敏[1] 魏伟 

机构地区：[1]暨南大学附属第一医院,广州510630 [2]中国广东脐血库,广州510663

出　　处：《生殖与避孕》2014年第2期108-115,共8页Reproduction and Contraception

摘　　要：目的:探讨人脐带Wharton’s间充质干细胞(Wharton’s jelly-derived mesenchymal stem cells,WJMSCs)在输卵管炎性不孕症治疗中的应用前景。方法:成年雌性Wistar大鼠50只,随机分为输卵管炎症模型组(n=40)和对照组(n=10)。其中,模型组大鼠双侧输卵管注入混合菌后再随机分成5组,急性治疗前组(n=5),于造模后第20日取材;急性治疗组(n=10),于造模第20日,给予腹腔注射WJMSCs(1×106/ml),7 d/次,共3次,疗程结束1周后5只取材;慢性治疗前组(n=5),于造模后30 d取材;慢性治疗组(n=10),于造模第30日开始给予WJMSCs;方法同急性治疗组,疗程结束1周后5只取材;慢性模型组(n=10),造模后不给予任何处理,第51日5只取材,所有余下雌鼠分别与雄鼠同笼。检测比较急、慢性输卵管炎模型大鼠腹腔注射WJMSCs治疗前、后血清INF-γ和TNF-α水平、输卵管超微结构、单位面积输卵管腔上皮皱褶长度(Lu)和分泌颗粒的密度(D),以及各组受孕率及产仔数。结果:急性输卵管炎期,输卵管上皮被破坏,间质水肿,治疗后基本恢复正常。慢性输卵管炎期,上皮扁平化,黏膜下纤维化,治疗后无明显改善。急性输卵管炎治疗前组Lu值无明显变化,而D值显著下降,治疗后恢复正常;慢性输卵管炎治疗前组Lu、D值均明显降低,治疗后无明显好转。急、慢性输卵管炎治疗前组血清TNF-α和INF-γ水平均明显增高,治疗后急性组恢复到正常水平,而慢性治疗组虽有下降,但仍高于正常水平。急性输卵管炎治疗组受孕率、产仔数恢复正常。慢性输卵管炎治疗前、后,其受孕率、产仔数均明显下降。结论:WJMSCs腹腔注射治疗大鼠急性输卵管炎,能降低血清炎症因子,恢复输卵管结构和功能,恢复生育功能,但对慢性输卵管炎的治疗效果不明显,提示在急性期治疗输卵管炎有可能防止继发不孕的发生。Objective： To explore the application prospect of Wharton＇s jelly-derived mesenchymal stem cells （WJMSCs） in infertility due to salpingitis. Methods： Totally 50 female Wistar adult rats were randomly divided into a normal group including 10 rats and a chronic salpingitis model group including 40 rats. Mixed bacteria were injected into bilateral fallopian tubes of the model group which was further divided into 5 groups at random. Model rats were extracted 20 d after modeled in the ex-treated acute group （n=5）; in the treated acute group （n=10）： from the 20th modeling day, modeled rats received 3 courses （a course of 7 d） of intraperitoneal injection of 1 ~ 106/ml WJMSCs and extracted one week afterwards; for the ex-treated chronic group （n=5）： rats were extracted 30 d after modeled; for the treated chronic group （n=10）： rats were given the same treatment as that of treated acute group from the 30th modeling day on and extracted in a week later; 5 rats were given no processing and extracted on the 5 lth day after modeled in the chronic model group （n=10）. The remaining rats mated with male rats. Serum INF-γand TNF-α, fallopian tube ultrastructure, epithelial folds length （Lu） of unit oviduct area, secretory granule density （D）, pregnancy rate and litter size were all compared between model groups of acute and chronic salpingitis rats before and after intraperitoneal injection of WJMSCs. Results： Epithelial edema and rupture, cell edema were observed in acute group. The oviduct epithelial structure returned to normal after treatment. In chronic group, the fallopian tube epithelium was changed into simple squamous epithelium, submucous fibrosis, and no significant improvement appeared after treatment. Lu showed no obvious changes and D value decreased remarkably in ex-treated acute group, and all returned to normal after treatment; Lu and D values of ex-treated chronic group were significantly reduced, but showed no significant improvement after treatment. TNF-α an

关 键 词：脐带Wharton’s间充质干细胞(WJMSCs) 输卵管炎 超微结构 炎症因子 

分 类 号：R711.76[医药卫生—妇产科学]