广州市哨点医院腹泻儿童隐孢子虫感染的分子流行病学研究  

Molecular epidemiological studies of Cryptosporidiosis diarrhea in children of Guangzhou sentinel hospital

作　　者：姚月娴[1] 陈惠芳[1] 刘谢[1] 效拟 肖媛[1] 黄燕惠[1] 赵德坚[1] 陈清[1] 俞守义[1] 

机构地区：[1]南方医科大学公共卫生与热带医学学院,广东广州510515

出　　处：《热带医学杂志》2014年第1期60-63,共4页Journal of Tropical Medicine

摘　　要：目的了解广州某哨点医院门诊腹泻儿童隐孢子虫感染现状和分子流行病学特征。方法收集2012年6月至2013年5月广州市某哨点医院门诊腹泻儿童的人口资料学和粪便标本,采用巢氏聚合酶链反应(Nested PCR)方法检测粪便标本中的隐孢子虫卵囊。结果348份粪便标本中隐孢子虫的检出率为3.45%(12/348),发病年龄以3岁以内为主(n=10),检出率在患者的性别、年龄、月份间差异均无统计学意义,时间分布有2个高峰,分别为3-4月和11-12月。检出的12株隐孢子虫经测序分型,8株为C.hominis,4株为C.parvum,其中Ia亚型6株,Ib亚型2株,IIa亚型3株,IId亚型1株。结论广州某哨点医院腹泻儿童存在隐孢子虫感染,高发于春季和冬季,以3岁以内婴幼儿为主,主要流行C.hominis。Objective The aim of this study was to understand the pathogenic prevalence and molecular epidemiology of Cryptosporidiosis diarrhea in children cases of hospital outpatients in Guangzhou. Methods Children outpatients with acute diarrhea were surveyed and their fecal specimens were collected at a sentinel hospital in Guangzhou from June 2012 to May 2013.Fecal samples were determined for Cryptosporidium by nested PCR. Results A total of 348 objects were investigated, with a positive rate of 3.45% （12/348）. Children below 3 year old had the highest rate of infection. The prevalence rates showed no differences among gender, age and time groups. Most infections occurred during the cool season of November to December,and the warm season of March to April. Twelve Cryptosporidium isolates were identified, of which 8 were Cryptosporidium hominis, and 4 Cryptosporidium parvum; among them 6 Ia, 2 Ib, 3 IIa, and 1 IId subtype. Conclusion Children diarrhea patients below age 3 of Guangzhou are at risk of Cryptosporidium infection in spring and winter.

关 键 词：腹泻 儿童 隐孢子虫病 分子流行病学 

分 类 号：R531.5[医药卫生—内科学]