在线电生Mn(Ⅲ)流动注射电化学发光法测定利血平  

Flow injection chemiluminescence determination of reserprine with Mn(Ⅲ) electrogenerated on line

作　　者：杨红兵[1] 张成孝[1] 

机构地区：[1]陕西师范大学化学系,陕西西安710062

出　　处：《陕西师范大学学报（自然科学版）》2000年第4期71-74,共4页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：陕西省自然科学基金资助项目! (98H0 3)

摘　　要：设计了一种用于流通体系电解池 ,将恒电流电解法与流动注射技术相结合 ,在线定量产生不稳定化学发光试剂Mn(Ⅲ ) ,使其浓度和反应进度得以在线控制 ,并基于Mn(Ⅲ )能够氧化利血平产生化学发光 ,建立了电生Mn(Ⅲ )化学发光法测定利血平的新方法 .其线性范围为 1 .0× 1 0 -7～ 1 .0× 1 0 -4 g/mL ,相对标准偏差为 2 .3%(c=5 .0× 1 0 -6g/mL ,n =1 1 ) ,该方法可用于针剂中利血平含量的测定 .A new flow injection chemiluminescence method is designed for the determination of reserprine based on a new chemiluminescence reaction between Mn(Ⅲ) and reserprine. The Mn(Ⅲ) is electrogenerated by constant current electrolysis on line. The linear range for reserprine is from 1.0×10 -8 ?g/mL to 1.0×10 -5 ?g/mL and the detection limit is 5.0×10 -9 ?g/mL. The relative standard deviation is 2.0%. The method has been used for the determination of reserprine in reserprine injections with satisfactory results.

关 键 词：电化学发光 流动注射分析 电解 MN(III) 利血平 药物分析 

分 类 号：R917[医药卫生—药物分析学] O657.3[医药卫生—药学]