不同浓度的GDNF对运动神经元诱向生长作用的研究  

Regeneration of motor neurons induced by different concentrations of GDNF in vitro

作　　者：夏思文[1,2] 陈世彩[1] 黄益灯 李孟[1] 李晓雨[1] 郑宏良[1] 

机构地区：[1]第二军医大学附属长海医院耳鼻咽喉头颈外科,上海200433 [2]解放军第118医院耳鼻咽喉头颈外科,浙江温州325000

出　　处：《山东大学耳鼻喉眼学报》2014年第1期10-13,共4页Journal of Otolaryngology and Ophthalmology of Shandong University

基　　金：国家自然基金面上项目(30672295/H1302);南京军区医学科技创新课题(10MA024)联合资助

摘　　要：目的探讨不同浓度的胶质源性神经营养因子(GDNF)对运动神经元的诱向生长作用。方法应用运动神经元体外诱向生长模型,将不同浓度的GDNF置于该模型中形成稳定的浓度梯度,72 h细胞培养后,通过测量模型中运动神经元生长锥的诱向生长的角度及神经元芽突的长度来初步筛选对运动神经元诱向作用最佳的GDNF浓度。结果 GDNF 200 ng/mL组与GDNF 100 ng/mL组间无明显区别(P>0.05);GDNF 200 ng/mL组及GDNF100 ng/mL组与空白对照组及GDNF 50 ng/mL组间比较,前两组均明显优于后两组,差异均具有统计学意义(P<0.05);空白对照组与GDNF 50 ng/mL组间差异明显(P<0.05)。结论 GDNF对运动神经元有明显的诱向生长作用,研究中初步确立了体外GDNF对运动神经元细胞诱向作用的最佳浓度为100 ng/mL。Objective To investigate the regeneration of motor neurons induced by different concentrations of glial cell line-derived neurotrophic factor （GDNF） in vitro to determine the optimal GDNF concentration. Methods With the motor neuron regeneration model, different concentrations of GDNF were added into the chamber and the formation of a stable concentration gradient was achieved. After 72-hour cell culture, the optimal concentration of GDNF to induce the regeneration was determined by measuring the angles and lengths of growth cones on motor neurons under different concentrations. Results For the regeneration of motor neurons, there was no significant difference between GDNF 200 ng/mL group and GDNF 100 ng/mL group （ P 〉 0.05 ）, however, both groups showed better effects than the blank control group and GDNF 50 ng/mL group （ P 〈 0.05 ）. The difference between the latter two groups was not significant （ P 〉 0.05 ）. Conclusion GDNF could induce the regeneration of motor neurons significantly, and the optimal concentration in vitro is 100 ng/mL.

关 键 词：胶质源性神经营养因子 运动神经元 诱向生长 浓度梯度 

分 类 号：R764.4[医药卫生—耳鼻咽喉科]