不同水流速度对中华倒刺鲃幼鱼自由基代谢的影响  

Effects of different water velocities on the free radical metabolism of juvenile Spinibarbus sinensis

作　　者：于丽娟[1] 李秀明[1,2] 易建华[1] 黄自豪[1] 陈冬明[1] 王志坚[1] 

机构地区：[1]西南大学淡水鱼类资源与生殖发育教育部重点实验室,水产科学重庆市市级重点实验室,重庆400715 [2]重庆师范大学进化生理与行为学实验室重庆市动物生物学重点实验室,重庆401331

出　　处：《中国水产科学》2014年第1期101-107,共7页Journal of Fishery Sciences of China

基　　金：重庆市自然科学基金项目(cstc2011jjA80012);重庆市科委重点实验室专项经费项目;重庆师范大学基金项目(12XLZ09)

摘　　要：在(25±0.5)℃下,设置4个水流速度实验组,分别为静水对照组0 bl/s(体长/秒)、1.0 bl/s组、2.0 bl/s组和4.0 bl/s组,研究不同水流速度对中华倒刺鲃(Spinibarbus sinensis)幼鱼肌肉自由基代谢的影响,训练周期为8周。结果显示,肌肉超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-PX)的活性随着水流速度的增加而升高,训练组过氧化氢酶(CAT)活性出现不同程度的下降,而抗氧化物谷胱甘肽(GSH)的含量未发生明显变化(P>0.05);训练组肌肉总抗氧化能力(T-AOC)均高于对照组,2.0 bl/s组最高,4.0 bl/s组次之,1.0 bl/s组最低,且2.0 bl/s和4.0 bl/s组T-AOC与对照组有显著性差异(P<0.05)。8周训练后,2.0 bl/s组丙二醛(MDA)含量显著低于其他3组(P<0.05),而H2O2含量随着水流速度增加呈上升趋势。8周运动训练对骨骼肌B细胞淋巴瘤因子-2(Bcl-2)、Bcl-2相关X蛋白(Bax)的含量及血清肌酸激酶(CK)活性无显著影响(P>0.05),但2.0 bl/s组Bax和Bax/Bcl-2显著低于4.0 bl/s组(P<0.05)。结果表明,2.0 bl/s的水流速度可提高中华倒刺鲃肌肉的抗氧化能力,降低脂质过氧化水平,并在一定程度上抑制肌细胞凋亡。Abstract： To explore the effects of exercise training under different velocities on the free radical metabolism of juvenile Spinibarbus sinensis, the experimental fish were divided into four groups at （25±0.5）°C. After the water current treat- ment （0 bl/s, 1.0 bl/s, 2.0 bl/s, 4.0 b1/s） for 8 weeks, the exercise training induced adaption of the antioxidant enzyme system of muscle, the activity of superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） increased with the increasing water velocity, while the activity of catalase （CAT） dropped in varying degrees. No significant differences in the level of glutathione（GSH） was observed among the treatments with various velocities. Compared with the control group, the content of the total antioxidant capacity （T-AOC） increased in the trained group, the maximum values were in the water velocity of 2.0 b1/s, the second maximum values were in 4.0 b1/s, with significant differences with control （P〈0.05）. At the end of experiment, the level of MDA in the group of 2.0 bus decreased significantly（P〈0.05）, however the content of H202 increased with the increasing water velocity. Compared with the control group, no significant dif- ferences in the content of Bcl-2, Bax and the activity of CK were found among treatments（P〉0.05）, however the ratio between Bax and Bcl-2 of 4.0 bus was significantly higher than that of 2.0 b1/s（P〈0.05）. The results showed that the exercise training under 2.0 bus could effectively improve the antioxidant function, decrease lipid peroxidation level, and inhibit muscle cell apoptosis of juvenile S. sinensis at some level.

关 键 词：流速 中华倒刺鲃 运动训练 自由基代谢 行为生态 

分 类 号：Q955[生物学—动物学]