羊皮革PCR鉴别方法研究  

Identification of Sheep Leather Using PCR Technology

作　　者：覃芳芳 王德莲 陈立伟[1,2] 孙世彧[1,2] 吴玉銮[1,2] 刘春生 罗海英 

机构地区：[1]广州市质量监督检测研究院,广东广州510100 [2]国家皮革制品质量监督检验中心( 广州),广东广州510100

出　　处：《中国皮革》2014年第3期44-47,共4页China Leather

基　　金：国家质检总局科研项目(2009QK372)

摘　　要：针对羊革,运用苯酚-氯仿2次纯化法成功地从羊革样品中获得质量相对较高的DNA,建立了羊革PCR的鉴定方法。该方法充分考虑了皮革材料的特殊性,利用裂解缓冲液释放皮革中的核酸,采用苯酚、氯仿混合溶液2次纯化的方法除去蛋白,最终获得纯化的皮革DNA。根据羊线粒体基因序列设计特异性的扩增引物,成功地从皮革样品DNA中扩增出294bp的羊内源基因片段,检测率达到62.5%。本方法通过分析皮革样品中的遗传学信息来鉴定皮革种类,特异性强、可作为皮革材质鉴别的一种手段。The high quality of sheep leather was successfully purified by the methods of phenol - chloroform secondary purifica- tion with PCR method. Fully taken account the particularity of leather material, the lysis buffer was used to release the nucleic acid in leather, then the phenol -chloroform mixed solution were twice applied to remove the protein in the lysis solution. PCR were performed using a pair of universal primers localized in the mitochondrial DNA of sheep. Fragments of about 294bp in size of mitochondrial DNA are amplified by PCR, the detection rate is 62.5 %. In general, the PCR method established is a kind of spe- cific detection technique for sheep leather identification.

关 键 词：羊革 皮革鉴别 脱氧核糖核酸 聚合酶链式反应 

分 类 号：TS57[轻工技术与工程—皮革化学与工程]