兔耳病理性瘢痕皮回植术后组织中基质金属蛋白酶－1及金属蛋白酶组织抑制剂－1表达的变化  

Changes in expression of matrix metalloproteinase-I （MMP-1） and tissue inhibitor of metailoproteinase-1（TIMP-I） in pathological scar tissue of rabbit ears after scar-skin replantation

作　　者：赵雪莲[1] 苏晓光[1] 张锤[1] 张春莉[1] 张卓男[1] 郑宝恒[1] 

机构地区：[1]石家庄,河北医科大学第二医院整形美容外科,石家庄050000

出　　处：《中华医学美学美容杂志》2014年第1期62-65,共4页Chinese Journal of Medical Aesthetics and Cosmetology

摘　　要：目的通过建立兔耳模型观察基质金属蛋白酶－1（matrixmetalloproteinase－1，MMP－1）及金属蛋白酶组织抑制剂－1（tissueinh｜bitorofmetalloproteinase1，TIMP1）在病理性瘢痕皮回植术后组织中表达的变化，探讨瘢痕皮回植治疗病理性瘢痕的机制。方法建立兔耳病理性瘢痕模型。共分为3组：正常皮肤组（对照组，A组）、病理性瘢痕组（B组）及瘢痕皮回植组（c组）。切取标本行HE染色和Masson特殊组织化学染色及免疫组织化学染色，观察各组标本MMP－1、TIMP－1的表达情况。结果病理性瘢痕经瘢痕皮回植术后，MMP－1及TIMP－l均较A组明显升高（P〈O．01）．MMP一1的表达较TIMP一1明显增强（P〈O．01）。结论瘢痕皮回植术治疗瘢痕的机制与瘢痕组织内MMP一1和TIMP一1相互作用的失衡有关。Objective To observe the changes of MMP-1 and TIMP-1 expression in the patho logical scar tissue after scar-skin replantation and to explore the mechanism of treating pathological scars with scar-skin replantation through a rabbit ear model. Methods Rabbit ears were used to es tablish the hypertrophic scar animal model in this study. Specimens were taken for three times： nor real skin, hypertrophic scar and scar skin replantion separately. We then performed HE staining, Masson staining and immunohistochemical staining to observe the expression of MMP 1 and TIMP-1 in these three groups of specimens. Results The expression of both MMP-1 and TIMP-1 significantly increased in the hypertrophic scar tissue after scar-skin replantation compared with the control group （P〈0.01）. The expression of MMP-1 increased more significantly than that of TIMP-1 （P〈0.01）. Conclusions The mechanism of scar-skin replantation＇s effect in the treatment for hypertrophic scar is relevant to the imbalance in the interaction between MMP 1 and TIMP-1 in the scar tissue.

关 键 词：增生性瘢痕 瘢痕皮回植术 病理性瘢痕 基质金属蛋白酶1 金属蛋白酶组 织抑制剂-1 

分 类 号：R622[医药卫生—整形外科]