大黄素对肝纤维化大鼠肺损伤的保护作用  

Protective effect of emodin on lung injury induced by hepatic fibrosis in rats

作　　者：张丽丽[1] 张慧英[1] 王黎敏[2] 李旭炯[3] 贾建桃[1] 吕敏丽[4] 范毅敏[2] 张翠英[3] 刘明社[5] 赵中夫[5] 韩德五[6] CHENG Ji 

机构地区：[1]长治医学院病理生理学教研室,山西长治046000 [2]长治医学院机能实验室,山西长治046000 [3]长治医学院生理学教研室,山西长治046000 [4]山西医科大学第二医院ICU,山西太原030001 [5]长治医学院肝病研究所,山西长治046000 [6]山西医科大学肝病研究所,山西太原030001 [7]南加州大学Keck医学院肝病研究中心,美国加利福尼亚州洛杉矶90089

出　　处：《中国病理生理杂志》2014年第2期291-296,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金(No.81070339);山西省国际科技合作计划(No.2010081068);山西医科大学细胞生理学省部共建教育部重点实验室主任基金资助项目(No.2010-09);山西省回国留学人员科研基金资助项目(No.211-091)

摘　　要：目的:探讨不同剂量大黄素对肝纤维化大鼠肺损伤的保护作用。方法:采用复合致病因素法(CCl4、乙醇、高脂、高胆固醇和低胆碱)建立肝纤维化大鼠模型并以不同剂量(20 mg/kg和40 mg/kg)大黄素进行治疗。4周后,测定肝指数,检测血清内毒素、同型半胱氨酸、反映肝功能的指标白蛋白、天门冬氨酸氨基转移酶、丙氨酸氨基转移酶、总胆红素、总胆固醇、甘油三酯和肝纤维化指标透明质酸、层黏连蛋白、Ⅳ型胶原蛋白、Ⅲ型前胶原蛋白的含量,观察肝组织病理学改变;测定肺指数,光镜下行肺组织病理学观察,检测肺组织匀浆肿瘤坏死因子α(TNF-α)、丙二醛(MDA)、一氧化氮(NO)和过氧亚硝基阴离子(ONOO-)含量。结果:大鼠肝纤维化模型复制成功,模型组大鼠肺指数明显增加,肺脏发生水肿、炎症反应,肺匀浆TNF-α、MDA、NO和ONOO-含量明显增加;大黄素治疗组肺指数较模型组下降,肺组织病理性损伤明显减轻,肺组织TNF-α、MDA、NO和ONOO-含量明显降低。结论:大黄素对肝纤维化大鼠的肺损伤具有一定的保护作用。AIM： To explore the protective effect of emodin on lung injury induced by hepatic fibrosis in rats. METHODS： The hepatic fibrosis rat model was established with multiple pathogenic factors （CCl4, ethanol, high fat, high cholesterol and low choline） and treated with different doses （20 mg/kg and 40 mg/kg） of emodin for 4 weeks. The hepatic index was measured. The biochemical indexes, endotoxin, homocysteine, albumin, aspartate aminotransferase,ala- nine aminotransferase, total bilirubin, total cholesterol and triglyceride, and hepatic fibrosis indexes, hyaluronic acid, laminin, collagen Ⅳ and procollagen Ⅲ, were detected. The histopathological changes of the liver were observed. The pul- monary index was determined. The histopathological changes of the lungs were observed. The levels of tumor necrosis factor α （TNF-α）, malondialdehyde （ MDA）, nitric oxide （NO） and peroxynitrite （ ONOO^- ） in the lung tissues were analyzed. RESULTS： The rat hepatic fibrosis model was successfully established. In model group, lung edema and inflammation oc- curred, and the pulmonary index and the levels of TNF-α, MDA, NO and ONOO^- in the lung tissues were increased sig- nificantly. In emodin treatment groups, the pulmonary indexes were lower than that in model group. The pathological injury of the lung tissues was alleviated. The levels of TNF-α, MDA, NO and ONOO^- in the lung tissues were decreased. CON- CLUSION： Emodin has a protective effect on lung injury induced by hepatic fibrosis in rats.

关 键 词：大黄素 肝纤维化 肝肺综合征 肺损伤 

分 类 号：R363[医药卫生—病理学]